Browsing by Author "Coucelo, Josefina"
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- Fish ultrasonography characterization of cardiac morphology and blood flowPublication . Coucelo, Josefina; Joaquim, N.; Aníbal, J.; Coucelo, JosefinaNon invasive identification and characterization of cardiac morphology and flow of Lophius s.p. and Halobactrachius s.p. was performed using color echo doppler. We used an ALOKA echo camera SSD 830 with a 5 MHZ transducer. Fishes were kept alive with water circulation device. The transducer was applied in the ventral middle line over heart chambers. Bidimensional images were obtained from long and short axis incidences at various levels of cardiac chambers. During each cardiac cycle and for every specimen it was possible to identify the relationship between each structure, measure longitudinal and transversal diameters, identify blood ?uxes and quantify their velocities. Conclusion: In live specimens it is possible, applying non invasive techniques, to observe the cardiac structures and to study the kinetic of the ventricle and bulbus, to identify blood ?ow and to measure his velocity. This can be used as an experimental model for cardiac function.
- Polimorfismo genético da haptoglobina e grau de gravidade da hipertensão arterial essencialPublication . Monteiro, C.; Lança, H.; Joaquim, N.; Lamy, I.; Aníbal, J.; Coucelo, Josefina; Azevedo, J.; Bicho, M.É conhecida a associação do polimorfismo genético da haptoglobina(Hp)quer com a sensibilidade ao sódio da pressão arterial quer com a gravidade hipertensão arterial (HTA).
- Polimorfismo genético da haptoglobina e grau de gravidade da hipertensão arterial essencialPublication . Monteiro, C.; Lança, H.; Joaquim, N.; Lamy, I.; Aníbal, J.; Coucelo, Josefina; Azevedo, J.; Bicho, M.A susceptibilidade genética para a hipertensão arterial envolve processos nos diferentes passos da sua história natural que podem estar activos em indivíduos e/ou famílias. Nesta susceptibilidade consideram-se o desenvolvimento da doença o seu curso clínico em termos de gravidade e complicações e ainda a variação da resposta terapêutica.
- Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administrationPublication . S. Soares, Sandra; Martins, H.; Duarte, Rui O.; Moura, José J. G.; Coucelo, Josefina; Gutiérrez-Merino, Carlos; Aureliano, M.The contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1 mM total vanadium) in Sparus aurata. Although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. After 1, 6 and 12 h upon decavanadate administration the increase of vanadium in blood plasma, red blood cells and in cardiac mitochondria and cytosol is not affected in comparison to the administration of a metavanadate solution containing labile oxovanadates. Cardiac tissue lipid peroxidation increases up to 20%, 1, 6 and 12 h after metavanadate administration, whilst for decavanadate no effects were observed except 1 h after treatment (+20%). Metavanadate administration clearly differs from decavanadate by enhancing, 12 h after exposure, mitochondrial superoxide dismutase (SOD) activity (+115%) and not affecting catalase (CAT) activity whereas decavanadate increases SOD activity by 20% and decreases ( 55%) mitochondrial CAT activity. At early times of exposure, 1 and 6 h, the only effect observed upon decavanadate administration was the increase by 20% of SOD activity. In conclusion, decavanadate has a different response pattern of lipid peroxidation and oxidative stress markers, in spite of the same vanadium distribution in cardiac cells observed after decavanadate and metavanadate administration. It is suggested that once formed decameric vanadate species has a different reactivity than vanadate, thus, pointing out that the differential contribution of vanadium oligomers should be taken into account to rationalize in vivo vanadate toxicity.