Browsing by Author "Fernandes, F."
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- In vitro spore germination of Polystichum drepanum, a threatened fern from Madeira IslandPublication . Chá-Chá, R.; Fernandes, F.; Romano, AnabelaPolystichum drepanum (Sw) C. Presl is a threatened fern endemic to a few forest areas in the north-west of Madeira Island. The aims of this work were to establish suitable culture conditions for in vitro germination of spores, and to evaluate short-term storage conditions for P drepanum spores. The highest frequency of germination was obtained in Murishage and Skoog (MS) liquid medium, without agitation. However, gametophytes maintained in MS liquid medium did not grow and, after 4 weeks, became anoxic and died. Thus, after germination in liquid medium, gametophytes were transferred to an MS double-phase culture system for further growth. The effects of storage period, temperature, and relative humidity during storage on in vitro spore germination were studied. Spore viability was assessed after 2, 4 and 6 months, and high viability (> 94%) was observed in all the assays. However, germination capability decreased with increased storage periods. The number of sporophytes obtained also decreased with prolonged storage periods. The results indicate that spores of R drepanum stored for 4 months at 21 degrees C maintain high viability and high germination frequency. ne sporophytes obtained were acclimatised in a mixture of peat and vermiculite [2:1 (v/v)] under high relative humidity (90-95%). Seventy-five sporophytes were successfully acclimatised to ex vitro conditions and showed active growth in the glasshouse.
- Permanent genetic resources added to molecular ecology resources database 1 May 2009-31 July 2009Publication . Almany, G. R.; De Arruda, M. P.; Arthofer, W.; Atallah, Z. K.; Beissinger, S. R.; Berumen, M. L.; Bogdanowicz, S. M.; Brown, S. D.; Bruford, M. W.; Burdine, C.; Busch, J. W.; Campbell, N. R.; Carey, D.; Carstens, B. C.; Chu, K. H.; Cubeta, M. A.; Cuda, J. P.; Cui, Z.; Datnoff, L. E.; D'Ávila, J. A.; Davis, E. S.; Davis, R. M.; Diekmann, O. E.; Eizirik, E.; Fargallo, J. A.; Fernandes, F.; Fukuda, H.; Gale, L. R.; Gallagher, E.; Gao, Y.; Girard, P.; Godhe, A.; Gonçalves, E. C.; Gouveia, Licínia; Grajczyk, A. M.; Grose, M. J.; Gu, Z.; Halldén, C.; Härnström, K.; Hemmingsen, A. H.; Serrão, EsterThis article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.
- Species distribution models and mitochondrial DNA phylogeography suggest an extensive biogeographical shift in the high-intertidal seaweed Pelvetia canaliculataPublication . Neiva, J.; Assis, J.; Fernandes, F.; Pearson, G. A.; Serrão, EsterAim: Species distributions have been continuously adjusting to changing climatic conditions throughout the glacial–interglacial cycles. In the marine realm, evidence suggests that latitudinal range shifts, involving both spatial expansions and trailingedge contractions, may represent a common response to climatic oscillations. The biogeographical histories of coastal organisms, however, have been inferred primarily using molecular markers, potentially overlooking past range dynamics beyond contemporary rear edges. In this study we combined species distribution models (SDMs) and mitochondrial DNA (mtDNA) data to investigate the biogeographical history of the high-intertidal seaweed Pelvetia canaliculata. We investigated the hypotheses that its distribution is set by both marine and terrestrial climates and that its range has shifted northwards since the Last Glacial Maximum. Location North-eastern Atlantic intertidal from Portugal to Norway. Methods: In total, 432 individuals at 27 sites covering the extant range of Pelvetia canaliculata were sampled and sequenced for a c. 500 bp mtDNA intergenic spacer. A niche model was developed using marine and terrestrial variables. Range dynamics were reconstructed based on the geographical patterns of genetic variation and on the SDM projections for the Last Glacial Maximum (LGM) and the present. Results: The best distribution models incorporated both marine and terrestrial variables. LGM projections revealed suitable habitat between southern Morocco and the periglacial shorelines of the Celtic Sea. Pelvetia canaliculata exhibited a highly structured phylogeography, being subdivided into three largely disjunct lineages, two of them endemic to Iberia. The central/northern European lineage exhibited the highest haplotypic diversity and showed a consistent decline in nucleotide diversity and haplotypic richness at higher latitudes. Main conclusions: Assuming species/climate equilibrium, SDMs supported the hypothesis of a post-glacial latitudinal range shift. Molecular variation revealed contrasting demographic behaviours in Iberian and periglacial regions. In Iberia the low haplotypic diversity suggested complex range dynamics that are not fully captured by SDM projections. Periglacial regions, identified as the source of poleward colonization, were inferred to have been comparatively more stable. Greater attention should be paid to marine range dynamics at low-latitude range margins, particularly in genetically structured low-dispersal species exhibiting southern endemic variation.