Browsing by Author "Galanty, Agnieszka"
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- Determination of essential minerals and trace elements in edible sprouts from different botanical families—application of chemometric analysisPublication . Dobrowolska-Iwanek, Justyna; Zagrodzki, Paweł; Galanty, Agnieszka; Fołta, Maria; Kryczyk-Kozioł, Jadwiga; Szlósarczyk, Marek; Rubio, Pol Salvans; Saraiva de Carvalho, Isabel; Paśko, PawełBackground: elemental deficiency may result in the malfunctioning of human organisms. Sprouts, with their attractive looks and well-established popularity, may be considered as alternative sources of elements in the diet. Moreover, the uptake of micro- and macronutrients from sprouts is better when compared to other vegetable sources. The aim of the study was to determine and compare the level of the selected essential minerals and trace elements in 25 sprouts from different botanical families, to preselect the richest species of high importance for human diets. Methods: the Cu, Zn, Mn, Fe, Mg, Ca determinations were performed using atomic absorption spectrometry with flame atomization and iodine by the colorimetric method. Results: beetroot sprouts had the highest levels of Zn, Fe, and Mg, while onion sprouts were the richest in Mn and Ca, among all of the tested sprouts. Sprouts of the Brassicaceae family were generally richer in Ca, Mg, and Zn than sprouts from the Fabaceae family. Results allow preselection of the most perspective sprouts as possible dietary sources of essential minerals and trace elements. For rucola, leeks, onions, and beetroot sprouts, the data on minerals and trace element compositions were performed for the first time.
- Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp sylvestris) Harvested in South PortugalPublication . Makowska-Was, Justyna; Galanty, Agnieszka; Gdula-Argasinska, Joanna; Tyszka-Czochara, Malgorzata; Szewczyk, Agnieszka; Nunes, Ricardo; Carvalho, Isabel S.; Michalik, Marta; Pasko, PawelThis study has been aimed at providing a qualitative and quantitative evaluation of selected phytochemicals such as phenolic acids, flavonoids, oleuropein, fatty acids profile, and volatile oil compounds, present in wild olive leaves harvested in Portugal, as well as at determining their antioxidant and cytotoxic potential against human melanoma HTB-140 and WM793, prostate cancer DU-145 and PC-3, hepatocellular carcinoma Hep G2 cell lines, as well as normal human skin fibroblasts BJ and prostate epithelial cells PNT2. Gallic, protocatechuic, p-hydroxybenzoic, vanillic acids, apigenin 7-O-glucoside, luteolin 7-O-glucoside, and rutin were identified in olive leaves. The amount of oleuropein was equal to 22.64 g/kg dry weight. (E)-Anethole (32.35%), fenchone (11.89%), and (Z)-3-nonen-1-ol (8%) were found to be the main constituents of the oil volatile fraction, whereas palmitic, oleic, and alpha-linolenic acid were determined to be dominating fatty acids. Olive leaves methanol extract was observed to exerted a significant, selective cytotoxic effect on DU-145 and PC-3 cell lines. Except the essential oil composition, evaluated wild olive leaves, with regard to their quantitative and qualitative composition, do not substantially differ from the leaves of other cultivars grown for industrial purposes and they reveal considerable antioxidant and cytotoxic properties. Thus, the wild species may prove to be suitable for use in traditional medicine as cancer chemoprevention.
- In vitro biological activity screening of arbutus unedo leaves in light of their traditional usePublication . Nunes, Ricardo; Saraiva De Carvalho, Isabel; Paśko, Paweł; Tyszka – Czochara, Małgorzata; Szewczyk, Agnieszka; Szlósarczyk, Marek; Podolak, Irma; Galanty, AgnieszkaThe biological activity of A. unedo leaves, in relation to their traditional medicinal use in treating gastrointestinal and urinary tract diseases was verified. The ethanol extract of the leaves was quantitatively described in terms of flavonoids and phenolic acids (RP-HPLC), and zinc (voltametric analysis) and subjected to cytotoxic (MTT), antioxidant (TPC, TFC, TAA, RP, DPPH, and FRAP), antibacterial (disc diffusion), and antidiabetic assays (enzyme inhibition) in vitro. The extract was highly cytotoxic against prostate cancer cells, with less impact on colon carcinomas, while being nontoxic to normal prostate and kidney cells, and demonstrated antioxidant and antidiabetic activities.