Browsing by Author "Galibert, F."
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- Genomic resources for the aquaculture of European sea bassPublication . Volckaert, F.; Batargias, C.; Bonhomme, F.; Canario, Adelino V. M.; Chistiakov, D.; Choudhuri, J. V.; Galibert, F.; Georgoudis, A.; Haley, Chris; Hellemans, Bart; Kuhl, H.; Kotoulas, Georgios; Law, A.; Libertini, A.; Magoulas, A.; McAndrew, B. J.; Reinhardt, Richard; Senger, Fabrice; Souche, E.; Tsigenopoulos, C.; Whitaker, H. A.The BASSMAP consortium, funded by the European Union, has been set up to improve the understanding of the genome of European sea bass (Dicentrarchus labrax). The specific aim is to locate genes of known function on the physical map and to compare specific regions among perciforms. We have produced an F1 cross of outbred sea bass as mapping panel and a Bacterial Artificial Chromosome library (7× redundancy and 165 kb average insert size). End sequencing of the BAC library is in progress and a radiation hybrid panel is under construction.
- The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L)Publication . Senger, Fabrice; Priat, Catherine; Hitte, Christophe; Sarropoulou, Elena; Franch, Rafaella; Geisler, Robert; Bargelloni, Luca; Power, Deborah; Galibert, F.Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RHpanel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a costeffective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.