Browsing by Author "Horta, Soraia Cristina Filipe"
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- Development of an indirect ELISA against Equine Herpesvirus Types 1 and 4Publication . Horta, Soraia Cristina Filipe; Fonseca, Filomena; Mourão, MargaridaThe equine herpesviruses are important pathogens in the animal health context, namely in the equine’s health. So far, nine types have been identified, of which herpesvirus type 1 (EHV-1) and type 4 (EHV-4) are clinically, economically, and epidemiologically the most relevant due to the pathologies associated. The main objective of this work was to develop a peptide-based enzyme-linked immunosorbent assay (ELISA) for discrimination between EHV-1 and EHV-4. Selecting from the literature the immunogenic regions of both viruses, the tests were performed using a portion of glycoprotein E and a portion of glycoprotein G from each virus type. The results obtained until the conclusion of this work are promising, however, they were not conclusive. Nevertheless, they open the way for future testing, in order to make these ELISA test applicable for the detection of antibodies against EVH-1 and EHV-4.