Browsing by Author "Houard, S."
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- Selective detection of human papilloma virus DNAs by specific synthetic DNA probesPublication . Cravador, A.; Herzog, A.; Houard, S.; D'ippolito, P.; Carroll, R.; Bollen, A.Specific oligodeoxynucleotide probes ranging from 20 to 35 nucleotides were defined to differentiate each of the HPV1a, 5, 6b, 8, 11, 16, 18 and 33. They were chosen using computer programs developed to compare simultaneously several 8000 bp long DNA sequences. Sequences common to all and to specific groups of the HPV DNA were also selected. Specificity of 32P-labelled probes for HPV6b, 11, 16, 18 and 33 was demonstrated and the sensitivity of the assays was evaluated by filter hybridization with viral clones and with DNA from cervical tumor biopsies. © 1989.
- Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNAPublication . De Vos, M.J.; Cravador, A.; Lenders, J. P.; Houard, S.; Bollen, A.Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared. The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotin-labelled 29-mer oligodeoxynucleotide entirely prepared on solid support.