Browsing by Issue Date, starting with "1989"
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- Selective detection of human papilloma virus DNAs by specific synthetic DNA probesPublication . Cravador, A.; Herzog, A.; Houard, S.; D'ippolito, P.; Carroll, R.; Bollen, A.Specific oligodeoxynucleotide probes ranging from 20 to 35 nucleotides were defined to differentiate each of the HPV1a, 5, 6b, 8, 11, 16, 18 and 33. They were chosen using computer programs developed to compare simultaneously several 8000 bp long DNA sequences. Sequences common to all and to specific groups of the HPV DNA were also selected. Specificity of 32P-labelled probes for HPV6b, 11, 16, 18 and 33 was demonstrated and the sensitivity of the assays was evaluated by filter hybridization with viral clones and with DNA from cervical tumor biopsies. © 1989.
- Estrategias dramáticas al servicio de un punto de vista político: el romance «Quéxome de vos, el rey»Publication . Ferré, PereMe propongo hablar precisamente de un tema romancístico cuya redacción se hizo muy cerca del suceso histórico que la motivó. Me refiero el romance «Quéxome de vos el rey», en cuyo texto se resumen, con enorme precisión, informaciones relacionadas con la muerte del Duque de Braganza, D. Fernando, ordenada por D. Juan II de Portugal, en 1483, y que se efectuó, en Évora, el 28 de agosto de 1484.
- The detection of thin structures in imagesPublication . Spann, M.; Horne, C.; du Buf, J. M. H.An algorithm is presented which extracts thin regions from images. The algorithm requires no a priori knowledge about the region direction, exact width, phase etc. Results on a range of natural imagery are presented. © 1989.
- Porcine D-amino acid oxidase: production of the biologically active enzyme in Escherichia coliPublication . Ciccarelli, E.; Massaer, M.; Guillaume, J. P.; Herzog, A.; Loriau, R.; Cravador, A.; Jacobs, P.; Bollen, A.DNA molecules coding either for mature porcine D-amino acid oxidase or for truncated forms of the enzyme have been obtained by stepwise addition of synthetic oligonucleotides to a partial cDNA. Under the control of the λ P(L) thermoregulatable promoter, these DNAs were respectively expressed in Escherichia coli as 36, 28 and 25 kilodalton polypeptides, specifically recognised by antibodies raised against the natural enzyme. None of the truncated proteins were biologically active whereas the mature recombinant species was able to hydrolyze D-alanine in vitro as efficiently as the natural product.
- Autour du romanceiro portugais (1828-1988)Publication . Ferré, PereOn ne se propose point, dans les quelques pages suivant, d'ébaucher une histoire des cent soixante ans de tradition orale moderne des romances (brefs poèmes narratifs nés au XIV siècle, dérivant, structurellement, de la vieille épopée castillane et qui constituent la branche ibérique de la ballade européenne médiévale), qui a oscillé entre l'accueil le plus euphorique et la plus sceptique des attitudes, l'oubli.
- A escultura Barroca AlgarviaPublication . Lameira, Francisco; Correia, José Eduardo HortaO presente estudo tem as suas raízes mais profundas em 1983, ano em que o Dr. Álvaro Vieira Simões, meu antigo professor de várias cadeiras de História da Arte na Faculdade de Letras de Lisboa me incentivou e orientou na listagem de identificação, ordenamento e classificação do espólio de obras de talha e escultura na cidade de Faro desde os finais do século XVI até ao primeiro quartel do século XIX..
- Production of human recombinant proapolipoprotein A-I in Escherichia coli: purification and biochemical characterizationPublication . Moguilevsky, N.; Roobol, C.; Loriau, R.; Guillaume, J. P.; Jacobs, P.; Cravador, A.; Herzog, A.; Brouwers, L.; Scarso, A.; Gilles, P.; Homquist, L.; Carlson, L. A.; Bollen, A.A human liver cDNA library was used to isolate a clone coding for apolipoprotein A-I (Apo A-I). The clone carries the sequence for the prepeptide (18 amino acids), the propeptide (6 amino acids), and the mature protein (243 amino acids). A coding cassette for the proapo A-I molecule was reconstructed by fusing synthetic sequences, chosen to optimize expression and specifying the amino-terminal methionine and amino acids -6 to +14, to a large fragment of the cDNA coding for amino acids 15-243. The module was expressed in pOTS-Nco, an Escherichia coli expression vector carrying the regulatable X P^ promoter, leading to the production of proapolipoprotein A-I at up to 10% of total soluble proteins. The recombinant polypeptide was purified and characterized in terms of apparent molecular mass, isoelectric point, and by both chemical and enzymatic peptide mapping. In addition, it was assayed in vitro for the stimulation of the enzyme lecithin: cholesterol acyltransferase. The data show for the first time that proapo A-I can be produced efficiently in E. coli as a stable and undegraded protein having physical and functional properties indistinguishable from those of the natural product.