Percorrer por autor "Huffman, Michael A."
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- Applying collocation and APRIORI analyses to chimpanzee diets: methods for investigating nonrandom food combinations in primate self‐medicationPublication . Freymann, Elodie; Coelho, João d'Oliveira; Hobaiter, Catherine; Huffman, Michael A.; Muhumuza, Geresomu; Zuberbühler, Klaus; Carvalho, SusanaIdentifying novel medicinal resources in chimpanzee diets has historically presented challenges, requiring extensive behavioral data collection and health monitoring, accompanied by expensive pharmacological analyses. When putative therapeutic self‐medicative behaviors are observed, these events are often considered isolated occurrences, with little attention paid to other resources ingested in combination. For chimpanzees, medicinal resource combinations could play an important role in maintaining well‐being by tackling different symptoms of an illness, chemically strengthening efficacy of a treatment, or providing prophylactic compounds that prevent future ailments. We call this concept the self‐medicative resource combination hypothesis. However, a dearth of methodological approaches for holistically investigating primate feeding ecology has limited our ability to identify nonrandom resource combinations and explore potential synergistic relationships between medicinal resource candidates. Here we present two analytical tools that test such a hypothesis and demonstrate these approaches on feeding data from the Sonso chimpanzee community in Budongo Forest, Uganda. Using 4 months of data, we establish that both collocation and APRIORI analyses are effective exploratory tools for identifying binary combinations, and that APRIORI is effective for multi‐ item rule associations. We then compare outputs from both methods, finding up to 60% agreement, and propose APRIORI as more effective for studies requiring control over confidence intervals and those investigating nonrandom associations between more than two resources. These analytical tools, which can be extrapolated across the animal kingdom, can provide a cost‐effective and efficient method for targeting resources for further pharmacological investigation, potentially aiding in the discovery of novel medicines.
- Pharmacological and behavioral investigation of putative self-medicative plants in budongo chimpanzee dietsPublication . Freymann, Elodie; Carvalho, Susana; Garbe, Leif A.; Ghazhelia, Dinda Dwi; Hobaiter, Catherine; Huffman, Michael A.; Muhumuza, Geresomu; Schulz, Lena; Sempebwa, Daniel; Wald, Florian; Yikii, Eguma R.; Zuberbühler, Klaus; Schultz, Fabien; Armel Jackson SeukepWild chimpanzees consume a variety of plants to meet their dietary needs and maintain wellbeing. While some plants have obvious value, others are nutritionally poor and/or contain bioactive toxins which make ingestion costly. In some cases, these nutrient-poor resources are speculated to be medicinal, thought to help individuals combat illness. In this study, we observed two habituated chimpanzee communities living in the Budongo Forest, Uganda, and collected 17 botanical samples associated with putative self-medication behaviors (e.g., bark feeding, dead wood eating, and pith-stripping) or events (e.g., when consumer had elevated parasite load, abnormal urinalysis, or injury). In total, we selected plant parts from 13 species (nine trees and four herbaceous plants). Three extracts of different polarities were produced from each sample using n-hexane, ethyl acetate, and methanol/water (9/1, v/v) and introduced to antibacterial and anti-inflammatory in vitro models. Extracts were evaluated for growth inhibition against a panel of multidrug-resistant clinical isolates of bacteria, including ESKAPE strains and cyclooxygenase-2 (COX-2) inhibition activity. Pharmacological results suggest that Budongo chimpanzees consume several species with potent medicinal properties. In the antibacterial library screen, 45 out of 53 extracts (88%) exhibited ≥40% inhibition at a concentration of 256 μg/mL. Of these active extracts, 41 (91%) showed activity at ≤256μg/mL in subsequent dose-response antibacterial experiments. The strongest antibacterial activity was achieved by the n-hexane extract of Alstonia boonei dead wood against Staphylococcus aureus (IC50: 16 μg/mL; MIC: 32 μg/mL) and Enterococcus faecium (IC50: 16 μg/mL; MIC: >256 μg/mL) and by the methanol-water extract of Khaya anthotheca bark and resin against E. faecium (IC50: 16 μg/mL; MIC: 32 μg/mL) and pathogenic Escherichia coli (IC50: 16 μg/mL; MIC: 256 μg/mL). We observed ingestion of both these species by highly parasitized individuals. K. anthotheca bark and resin were also targeted by individuals with indicators of infection and injuries. All plant species negatively affected growth of E. coli. In the anti-inflammatory COX-2 inhibition library screen, 17 out of 51 tested extracts (33%) showed ≥50% COX-2 inhibition at a concentration of 5 μg/mL. Several extracts also exhibited anti-inflammatory effects in COX-2 dose-response experiments.
- Tropical field stations yield high conservation return on investmentPublication . Eppley, Timothy M.; Reuter, Kim E.; Sefczek, Timothy M.; Tinsman, Jen; Santini, Luca; Hoeks, Selwyn; Andriantsaralaza, Seheno; Shanee, Sam; Fiore, Anthony Di; Setchell, Joanna M.; Strier, Karen B.; Abanyam, Peter A.; Mutalib, Aini Hasanah Abd; Abwe, Ekwoge; Ahmed, Tanvir; Ancrenaz, Marc; Andriantsimanarilafy, Raphali R.; Ang, Andie; Aureli, Filippo; Barrett, Louise; Beehner, Jacinta C.; Benítez, Marcela E.; Bezerra, Bruna M.; Bicca‐Marques, Júlio César; Bikaba, Dominique; Bitariho, Robert; Boesch, Christophe; Bolt, Laura M.; Boonratana, Ramesh; Butynski, Thomas M.; Canale, Gustavo R.; Chapman, Colin A.; Carvalho, Susana; Chetry, Dilip; Cheyne, Susan M.; Cords, Marina; Cornejo, Fanny M.; Cortés‐Ortiz, Liliana; Coudrat, Camille N. Z.; Crofoot, Margaret C.; Cronin, Drew T.; Dadjo, Alvine; Dakpogan, S. Chrystelle; Danquah, Emmanuel; Davenport, Tim R. B.; Jong, Yvonne A. de; Torre, Stella de la; Dempsey, Andrea; Dimalibot, Judeline C.; Dolch, Rainer; Donati, Giuseppe; Estrada, Alejandro; Farassi, Rassina A.; Fashing, Peter J.; Fernandez‐Duque, Eduardo; Silva, Maria J. Ferreira da; Fischer, Julia; Flores‐Negrón, César F.; Fruth, Barbara; Neba, Terence Fuh; Gamalo, Lief Erikson; Ganzhorn, Jörg U.; Garber, Paul A.; Gnanaolivu, Smitha D.; Gonder, Mary Katherine; Bi, Sery Ernest Gonedelé; Goossens, Benoit; Gordo, Marcelo; Guayasamin, Juan M.; Guzmán‐Caro, Diana C.; Halloran, Andrew R.; Hartel, Jessica A.; Heymann, Eckhard W.; Hill, Russell A.; Hockings, Kimberley J.; Hohmann, Gottfried; Hon, Naven; Houngbédji, Mariano G.; Huffman, Michael A.; Ikemeh, Rachel A.; Imong, Inaoyom; Irwin, Mitchell T.; Izar, Patrícia; Jerusalinsky, Leandro; Kalema‐Zikusoka, Gladys; Kaplin, Beth A.; Kappeler, Peter M.; Kivai, Stanislaus M.; Knott, Cheryl D.; Kolasartsanee, Intanon; Koops, Kathelijne; Kowalewski, Martin M.; Kujirakwinja, Deo; Kumar, Ajith; Le, Quyet K.; Lewis, Rebecca J.; Lin, Aung Ko; Link, Andrés; Loría, Luz I.; Lormie, Menladi M.; Louis, Edward E.; Lwin, Ngwe; Maisels, Fiona; Malaivijitnond, Suchinda; Marisa, Lesley; McCabe, Gráinne M.; McGraw, W. Scott; Mekonnen, Addisu; Méndez‐Carvajal, Pedro G.; Minhós, Tânia; Montgomery, David M.; Morelos‐Juárez, Citlalli; Morgan, Bethan J.; Morgan, David; Etingüe, Amancio Motove; Ndiaye, Papa Ibnou; Nekaris, K. Anne‐Isola; Nguyen, Nga; Nijman, Vincent; Nishuli, Radar; Norconk, Marilyn A.; Oklander, Luciana I.; Oktaviani, Rahayu; Ostner, Julia; Otali, Emily; Perry, Susan E.; Ramos, Eduardo J. Pinel; Porter, Leila M.; Pruetz, Jill D.; Pusey, Anne E.; Queiroz, Helder L.; Ramírez, Mónica A.; Randriatahina, Guy Hermas; Rasoanaivo, Hoby; Ratsimbazafy, Jonah; Ratsirarson, Joelisoa; Razafindramanana, Josia; Razafindratsima, Onja H.; Reynolds, Vernon; Rizaldi, Rizaldi; Robbins, Martha M.; Rodríguez, Melissa E.; Rosales‐Meda, Marleny; Sanz, Crickette M.; Sarkar, Dipto; Savage, Anne; Schreier, Amy L.; Schülke, Oliver; Segniagbeto, Gabriel H.; Serio‐Silva, Juan Carlos; Setiawan, Arif; Seyjagat, John; Silva, Felipe E.; Sinclair, Elizabeth M.; Smith, Rebecca L.; Spaan, Denise; Stewart, Fiona A.; Strum, Shirley C.; Surbeck, Martin; Svensson, Magdalena S.; Talebi, Mauricio; Tédonzong, Luc Roscelin; Urbani, Bernardo; Valsecchi, João; Vasey, Natalie; Vogel, Erin R.; Wallace, Robert B.; Wallis, Janette; Waters, Siân; Wittig, Roman M.; Wrangham, Richard W.; Wright, Patricia C.; Mittermeier, Russell A.Conservation funding is currently limited; cost-effective conservation solutions are essential. We suggest that the thousands of field stations worldwide can play key roles at the frontline of biodiversity conservation and have high intrinsic value. We assessed field stations’ conservation return on investment and explored the impact of COVID-19. We surveyed leaders of field stations across tropical regions that host primate research; 157 field stations in 56 countries responded. Respondents reported improved habitat quality and reduced hunting rates at over 80% of field stations and lower operational costs per km2 than protected areas, yet half of those surveyed have less funding now than in 2019. Spatial analyses support field station presence as reducing deforestation. These “earth observatories” provide a high return on investment; we advocate for increased support of field station programs and for governments to support their vital conservation efforts by investing accordingly.