Browsing by Author "Morais, Matilde"
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- Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPasePublication . Morais, Matilde; Aureliano, M.; Bebianno, Maria JoãoIn the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs.
- Toxic effects of cisplatin cytostatic drug in mussel Mytilus galloprovincialisPublication . Trombini, Chiara; da Fonseca, Taina Garcia; Morais, Matilde; Rocha, Thiago Lopes; Blasco, Julian; Bebianno, Maria JoãoAntineoplastic drugs used in chemotherapy were detected in aquatic environment: despite the very low concentrations (ng L-1 to ug L-1), due to their potent mechanism of action they could have adverse effects on non-target aquatic organisms particularly under chronic exposure. Cisplatin (CDDP) is one of the most effective anticancer drug currently in use but information on its ecotoxicological effects is very limited. In this study, Mytilus galloprovincialis was used to investigate the toxic effects related to CDDP exposure. Mussels were exposed to cisplatin (100 ng L-1) for 14 days: antioxidant (superoxide dismutase, catalase, total and selebium-dependent glutathione peroxidase) and phase II (glutathione-S-transferase) enzymes activities, oxidative damage (lipid peroxidation), genotoxicity (DNA damage) and neurotoxicity (acetylcholinesterase) was evaluated. Results indicate that CDDP at tested concentration induce changes in the antioxidant capacity, oxidative stress in target organs (digestive gland and gills) as well as DNA damage in mussel hemocytes and neurotoxicity representing a risk for non-target organisms. (C) 2016 Elsevier Ltd. All rights reserved.