Percorrer por autor "Senger, Fabrice"
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- A gene-based radiation hybrid map of the gilthead sea bream Sparus aurata refines and exploits conserved synteny with Tetraodon nigroviridisPublication . Sarropoulou, Elena; Franch, Rafaella; Louro, Bruno; Power, Deborah; Bargelloni, Luca; Magoulas, Antonio; Senger, Fabrice; Kotoulas, Georgios; Geisler, RobertBackground: Comparative teleost studies are of great interest since they are important in aquaculture and in evolutionary issues. Comparing genomes of fully sequenced model fish species with those of farmed fish species through comparative mapping offers shortcuts for quantitative trait loci (QTL) detections and for studying genome evolution through the identification of regions of conserved synteny in teleosts. Here a comparative mapping study is presented by radiation hybrid (RH) mapping genes of the gilthead sea bream Sparus aurata, a non-model teleost fish of commercial and evolutionary interest, as it represents the worldwide distributed species-rich family of Sparidae. Results: An additional 74 microsatellite markers and 428 gene-based markers appropriate for comparative mapping studies were mapped on the existing RH map of Sparus aurata. The anchoring of the RH map to the genetic linkage map resulted in 24 groups matching the karyotype of Sparus aurata. Homologous sequences to Tetraodon were identified for 301 of the gene-based markers positioned on the RH map of Sparus aurata. Comparison between Sparus aurata RH groups and Tetraodon chromosomes (karyotype of Tetraodon consists of 21 chromosomes) in this study reveals an unambiguous one-to-one relationship suggesting that three Tetraodon chromosomes correspond to six Sparus aurata radiation hybrid groups. The exploitation of this conserved synteny relationship is furthermore demonstrated by in silico mapping of gilthead sea bream expressed sequence tags (EST) that give a significant similarity hit to Tetraodon. Conclusion: The addition of primarily gene-based markers increased substantially the density of the existing RH map and facilitated comparative analysis. The anchoring of this gene-based radiation hybrid map to the genome maps of model species broadened the pool of candidate genes that mainly control growth, disease resistance, sex determination and reversal, reproduction as well as environmental tolerance in this species, all traits of great importance for QTL mapping and marker assisted selection. Furthermore this comparative mapping approach will facilitate to give insights into chromosome evolution and into the genetic make up of the gilthead sea bream.
- Genomic resources for the aquaculture of European sea bassPublication . Volckaert, F.; Batargias, C.; Bonhomme, F.; Canario, Adelino V. M.; Chistiakov, D.; Choudhuri, J. V.; Galibert, F.; Georgoudis, A.; Haley, Chris; Hellemans, Bart; Kuhl, H.; Kotoulas, Georgios; Law, A.; Libertini, A.; Magoulas, A.; McAndrew, B. J.; Reinhardt, Richard; Senger, Fabrice; Souche, E.; Tsigenopoulos, C.; Whitaker, H. A.The BASSMAP consortium, funded by the European Union, has been set up to improve the understanding of the genome of European sea bass (Dicentrarchus labrax). The specific aim is to locate genes of known function on the physical map and to compare specific regions among perciforms. We have produced an F1 cross of outbred sea bass as mapping panel and a Bacterial Artificial Chromosome library (7× redundancy and 165 kb average insert size). End sequencing of the BAC library is in progress and a radiation hybrid panel is under construction.
- The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L)Publication . Senger, Fabrice; Priat, Catherine; Hitte, Christophe; Sarropoulou, Elena; Franch, Rafaella; Geisler, Robert; Bargelloni, Luca; Power, Deborah; Galibert, F.Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RHpanel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a costeffective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.