Percorrer por autor "Singab, Abdel Nasser B."
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- Assessing the bioactive potential of Lysimachia atropurpurea extracts using HPLC-MS/MS, in vitro and in silico analysisPublication . Ak, Gunes; Nilofar, Nilofar; Saka, Enver; Uba, Abdullahi Ibrahim; Rodrigues, Maria João; Fernandes, Eliana; Custódio, Luísa; Yildiztugay, Evren; Yapıcı, Ismail; Gulcin, Ilhami; Mahmoud, Orchid A.; Eldahshan, Omayma A.; Singab, Abdel Nasser B.; Wu, Yimao; Li, Meng-Yao; Zengin, GokhanThe genus Lysimachia is of great interest to the scientific community, especially in terms of its potential anticancer effects. In this study, the aerial parts and roots of Lysimachia atropurpurea L. were collected and extracted by maceration using solvents of ethyl acetate (EA), ethanol (EtOH), ethanol/water, and water. The biological activities of the extracts, including antioxidant, enzyme inhibition, and anticancer effects, were evaluated using various assays. High-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) analysis revealed a total of 32 compounds in the extracts of L. atropurpurea. The roots showed significantly the highest antioxidant activity compared to the aerial part. In case of cholinesterase inhibition, the aerial parts of the EtOH extract showed the highest acetylcholinesterase (AChE) inhibition activity, measuring 3.05 mg galatamine equivalent (GALAE)/g. The EtOH and EtOH/water extracts exhibited the strongest cytotoxicity, reducing the viability of human neuroblastoma (SH-SY5Y) and human hepatocarcinoma (HepG2) cancer cells to as low as 4.86–6.33 %. The results of network pharmacology and molecular docking suggest that the extract of L. atropurpurea exerts inhibitory effects on hepatocellular carcinoma through the modulation of SRC, PI3K, and HSP90, while it demonstrates potential inhibitory activity against neuroblastoma by targeting SRC, PI3K, HSP90, ESR1, AKT, and other related targets. In conclusion, the L. atropurpurea extracts showed potential antioxidant, enzyme inhibition, and selective anticancer effects, which support their potential for further research as therapeutic agents in drug development.
- Functional constituents of Colchicum lingulatum Boiss. & Spruner subsp. Rigescens K. Perss. Extracts and their biological activities with different perspectivesPublication . Yagi, Sakina; Zengin, Gokhan; Eldahshan, Omayma A.; Singab, Abdel Nasser B.; Selvi, Selami; Cetiz, Mehmet Veysi; Rodrigues, Maria João; Custódio, Luísa; Dall’Acqua, Stefano; Elhawary, Esraa A.The genus Colchicum comprises medicinal plants with important bioactive alkaloids, mainly colchicine, and phenolics. The present study was aimed to determine for the first time the phytoconstituents, antioxidant, enzyme inhibition and cytotoxic properties of corms of C. lingulatum. Different solvents extracts (hexane, ethyl acetate, methanol and water) were obtained. The extracts were examined for chemical characterization, antioxidant, enzyme inhibition and cytotoxic properties. To gain more insights, in silico and network pharmacological analysis were performed. Results of GC/MS analysis of the hexane extract revealed that the extract was dominated by oxygenated diterpenes (37%). UHPLC/MS analysis of ethyl acetate, methanol and aqueous extracts indicated the presence of flavonoids as the most abundant class in addition to phenolic acids and alkaloids. The alkaloid colchicine together with its derivatives colchiceine, colchiciline and isomers were detected in the methanol and aqueous extracts. Results of antioxidant activity showed that the methanol extract exhibited the highest DPPH radical scavenging. At 100 mu g/mL, the ethyl acetate, methanol and aqueous extracts displayed potent cytotoxicity against the human embryonic (HEK 293), murine macrophages (RAW 264.7). In silico analyzes have investigated the potential of C. lingulatum in cancer therapy using network pharmacology and molecular docking methods. These results demonstrate C. lingulatum can be a valuable source of bioactive compounds in the development of functional applications including nutraceuticals.