Browsing by Author "Viegas, C. A."
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- AQR1 gene (ORF YNL065w) encodes a plasma membrane transporter of the major facilitator superfamily that confers resistance to short-chain monocarboxylic acids and quinidine in Saccharomyces cerevisiaePublication . Tenreiro, S.; Nunes, Patrícia A.; Viegas, C. A.; Neves, M. S.; Teixeira, M. C.; Cabral, M. G.; Sa Correia, I.We report results on the functional analysis of Saccharomyces cerevisiae ORF YNL065w, predicted to code for a protein belonging to the poorly characterized major facilitator superfamily (MFS) of transporters that are involved in multidrug resistance (MDR). YNL065w is important for a moderate increase of yeast tolerance to ketoconazole and to the cationic dye crystal violet; it protects the cell against short-chain monocarboxylic acids (C-2-C-6), but not against highly liposoluble acids such as octanoic acid or the phenoxyacetic-acid herbicides 2,4-D and MCPA; it is also a determinant of resistance to the antiarrhytmic and antimalarial drug quinidine. The encoding ORF was, thus, denominated the AQR1 gene. Results obtained using an AQR1-lacZ fusion indicate that gene expression is very low and it is not stimulated under weak acid stress. The encoded putative transporter was localized in the plasma membrane by fluorescence microscopy observation of the overproduced Aqr1-GFP fusion protein distribution. (C) 2002 Elsevier Science (USA).
- Comparative analysis of gene expression profiles among distantly related fish species. Insight into skeletal and immunological systemsPublication . Mariani, Valentina; Viegas, C. A.; Gavaia, Paulo J.; Cancela, Leonor; Giuffra, ElisabettaThe aim of this project is to identify and characterize the polymorphism of genes relevant for the physiology of skeletal and immunological tissues, and highly conserved across fish species. To achieve this goal, the expression profiles of an ancient species relevant for caviar and fillet production(A. transmontanus) are compared to available and in progress information (Marine Genomics network of Excellence, UE) of modern Teleosts.
- Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistryPublication . Gavaia, Paulo J.; Viegas, C. A.; Pinto, Jorge; Sarasquete, C.; Cancela, LeonorAlthough the presence of osteocalcin (BGP) has been known for a number years, little knowledge on the regulation of expression and tissue localization of this protein in lower vertebrate organisms. In this site we have investigated the site of BGP mRNA expression by in situ hybridization using radiolabeled riboprobes and the tissue localization of the mature protein by immunohistochemistry in the Senegal sole (Solea senegalensis), zebrafish (Danio rerio), and gilthead sea bream (Sparus aurata) using fish BGP polyclonal antibodies.