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- Functional expression of the human CHIP28 water channel in a yeast secretory mutantPublication . Laizé, Vincent; Rousselet, G.; Verbavatz, J. M.; Berthonaud, V.; Gobin, R.; Roudier, N.; Abrami, L.; Ripoche, P.; Tacnet, F.The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles, lmmunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.