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- Screening natural resources for mineralogenic and osteogenic bioactivities using in vitro and in vivo fish systemsPublication . Tarasco, Marco; Laizé, Vincent; Cancela, M. LeonorBone disorders affect millions of people worldwide, and available treatments have only a limited efficacy and/or bring undesirable side effects. There is therefore the need for novel compounds with bone anabolic properties. Due to its technical advantages, fish have been successfully used in biomedical and pharmaceutical research to screen for osteogenic compounds. The aim of this work was to evaluate mineralogenic/osteogenic performance of semi-purified fractions and purified molecules from terrestrial plants (cardoon and eucalyptus) and cyanobacteria. VSa13 cell line established from gilthead seabream (Sparus aurata) vertebra and capable of in vitro mineralization was used to assess the mineralogenic potential of the extracts, while zebrafish operculum was used to develop and establish a reliable in vivo model to screen molecules for osteogenic activity. Extracts from cardoon, eucalyptus and some cyanobacteria exhibited some moderate mineralogenic effect while triterpenic acids purified from eucalyptus strongly increased mineral deposition. Pro-mineralogenic compounds were further analysed for their bone anabolic action on zebrafish operculum and lipophilic cardoon extract, ursolic acid, oleanolic acid and ethyl acetate fraction of cyanobacteria strain #13 were found to promote an osteogenic effect. While the osteogenicity of ursolic acid has already been reported in mouse, the action of oleanolic acid and cyanobacteria extract on bone formation is revealed here for the first time. The use of a double transgenic line expressing fluorescent proteins under the control of osterix/sp7 and osteocalcin/oc2 promoters to get insights into mechanisms underlying the osteogenic activity of natural compounds has been explored. In vitro and in vivo data generated within the scope of this work have not only demonstrated the potential of natural resources to provide molecules with mineralogenic/osteogenic activity that may be used in pharmaceuticals or nutraceuticals but also the suitability of fish systems to screen for these molecules.
- Histórias com história dentroPublication . Serra, Sara Luísa Ascenção Marques Carvela; Gonçalves, Artur Henrique RibeiroO presente relatório pretende aprofundar conhecimentos acerca do contributo da transversalidade/transdisciplinaridade e monodocência em sala de aula. O trabalho define-se como um estudo de caráter qualitativo, realizado no âmbito da Prática de Ensino Supervisionado do curso de mestrado em Ensino do 1.º e 2.º Ciclo do Ensino Básico, em duas escolas do Agrupamento de Escolas Albufeira Poente, envolvendo duas turmas do 5.º e 6.º anos do ensino básico. Defino, como objetivo central de estudo e intervenção educativa, compreender a forma pela qual um docente de duas áreas disciplinares distintas, como é o caso do Português e da História e Geografia de Portugal, pode utilizar o mesmo recurso pedagógico nas duas disciplinas, simultaneamente, para lecionar conteúdos diferentes, permitindo-lhe, assim, uma maior e melhor flexibilidade curricular. O estudo assenta numa atividade em sala de aula, tendo sido definida uma estratégia de ação para os vários momentos de leitura. O recurso utilizado foi a obra literária de José Gomes Ferreira, as Aventuras de João Sem Medo. Panfleto mágico em forma de romance (1963), incluído no Plano Nacional de Leitura, envolvendo os alunos no processo enquanto atores ativos acerca da exequibilidade, ou não, do mesmo recurso para diferentes áreas disciplinares.