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Browsing by Issue Date, starting with "2024-06-01"

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  • Histological evaluation of purple sea urchin (Paracentrotus lividus) gonads: influence of temperature, photoperiod regimes, and diets
    Publication . Araújo, João; Gamboa, Margarida; Choulis, Ioannis; Mendes, Ana Candeias; Cabrita, Elsa; Pousão-Ferreira, Pedro; Soares, Florbela
    Understanding and controlling reproductive cycles and gonad maturation in cultivated species are crucial in aquaculture. The sea urchin Paracentrotus lividus, known for its edible gonads, requires careful maturation control for both reproduction and commercialization. This study explores the impact of temperature, photoperiod, and diet on gonad development to enhance year-round aquaculture practices. Using two independent cultivation systems, we manipulated environmental conditions to mimic different seasons. Sea urchins were exposed to natural or manipulated temperature and photoperiod conditions and fed either natural (Ulva spp.) or formulated diets. The gonadosomatic index (GI) and histological analysis were used to assess gonad development. The results revealed a clear correlation between environmental conditions, diet, and gonad maturation. Manipulated conditions accelerated maturation, with sea urchins showing advanced stages compared to natural conditions. Furthermore, sea urchins fed formulated diets exhibited higher GI values, indicating enhanced maturation. Histological analysis confirmed accelerated maturation, particularly in females. This study underscores the feasibility of controlling gonad maturation through environmental manipulation and diet, enabling year-round marketable gonad production. Providing formulated diets rich in polyunsaturated fatty acids, notably docosahexaenoic acid (DHA), enhances the commercial value of sea urchins. These findings optimize aquaculture practices for P. lividus, highlighting its adaptability to the maximal production of gonads throughout the year.
    2024-06-01Journal article Open Access Show more
  • Retro tIming: A multicentric retrospective analysis of immunotherapy timing in metastatic melanoma
    Publication . Gonçalves, Lisa; Goncalves, Duarte; Casanelles, Teresa Esteban; Guerra, Laura Pratas; Menezes, Maria B.; Branco, Vanessa Duarte; Luís, Joana Alves; Simões, Filipa; Gramaça, João; Pinho, Inês S.; Barroso, Tiago; Patel, Vanessa; Martins, Miguel Esperança; Brás, Raquel Lopes; Sousa, Rita Teixeira de; Mansinho, André; Torres, Sofia; Costa, Luis
    Immune checkpoint inhibitors (ICI) targeting programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) are now the standard of care in the treatment of metastatic melanoma (mM). The interplay between cancer cells and tumor microenvironment cells (e.g. immune cells) impacts cancer cell survival, local invasion, and metastatic dissemination. Chrono-immunotherapy is an emerging field as circadian oscillations are observed in the immune landscape (e.g immune cell numbers) as well as in the expression of immunotherapy targets (e.g. PD-1).
    2024-06-01Conference object Restricted Show more