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- Modelling left ventricular noncompaction cardiomyopathy in cardioidsPublication . Correia, Cátia Dias; Calado, Sofia; Bragança, JoséCongenital heart defects are the most common deficiency in new-borns. Specifically, a failure during myocardial compaction can lead to excessive trabeculation and intertrabecular recesses seen in left ventricular noncompaction (LVNC). Due to heterogeneity found in patients, more in-depth molecular research, with better disease models, is necessary to identify underlying genetic pathways and pathogenesis. Here, a novel self-assembling human cardioid differentiation protocol was used. Initially, three concentrations of a canonical Wnt pathway activator were tested, and addition of ascorbic acid. A higher concentration led to a full loss of organoids, while ascorbic acid slightly increased the number of organoids discarded but increased the number of beating control-derived organoids. Lastly, with hiPSCs established from LVNC patients, and a healthy relative, three initial seeding densities were tested. We experienced a high degree of heterogeneity in size and morphology, vascularisation formation, number of beating and discarded cardioids. We also analysed organoid circularity at two different stages of differentiation, but no conclusions can be made due to result variability and inconsistency between both cell lines, and/in all seeding conditions presenting no clear pattern. We also experienced a lack of fluorescence signal or a low antibody penetrability after immunofluorescence staining, and increased all incubation periods but still experienced a lack of signal for cardiomyocytes. Then, due to organoid size and density, we observed a low reachable sample depth during fluorescence microscopy. An additional step of ScaleA2 optical clearing was tested, which showed that a 10 day incubation period is crucial for imaging quality and reachable depth improvement. In conclusion, this is a promising but very demanding protocol that has to be thoroughly followed, with many influencing factors. More work is still necessary to improve its efficiency for further studies to determine their cardiac development capacity, and if it can be used to model LVNC in the future.
- Contributions to a theoretical-practical analysis of the efficiency of cine-imagetic typography in the reading experiencePublication . Filipe Martins, Nuno; Mendes da Silva, Bruno; Marques, Tiago NavarroThe thematic of this research, questions the imagistic quality of the written word and the inherent artistic conventions of combination of form, typography, and composition. The focus of this analysis is the significance of typographic compositions, in which words acquire an intentional visualization that can be “understood” even before verbal reading. To our analysis, we used a selection of poems from Sophia de Mello Breyner Andresen to question: How can we amplify semantics and emotional perception of a poem? To answer it, we used a practice-based research methodology defined as a/r/tography (Irwin 2013) and a/r/cography (Veiga 2020), articulated with interpretative phenomenology, ethnographic studies, case studies analysis, focus groups, artistic references analysis, visual research, user-centred research, that helped the construction of typographic laboratory to collect data. The result was the validation of the prototype typographic laboratory, with user experience data collection in an event with a real audience which enhanced the experience of the final interactive typographic installation. The contribution of this research is through the visual interpretation of poems, to create a media-art experience that defies an audience to perceive it and interact in various ways and to the creation of knowledge about imagistic typographic approaches, the use of kinetic an interactive behaviour, to amplify the significance of static text, in ways that can be influential trough the mediation of the reading experience.