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- Establishment of an in vitro propagation protocol for Thymus lotocephalus, a rare aromatic species of the Algarve (Portugal)Publication . Coelho, Natacha; Gonçalves, Sandra; González-Benito, María Elena; Romano, AnabelaThe aim of this work was to develop an in vitro propagation protocol for the endangered species Thymus lotocephalus using seedlings as explants. Several macronutrient concentrations of Murashige and Skoog medium (MS), cytokinin types and concentrations, and cytokinin/auxin combinations were tested to assess the shoots’ proliferation capacity. Although the best proliferation results were obtained with 6-benzyladenine, high percentages of hyperhidric shoots were observed. Because high proliferation of healthy shoots was observed in MS medium that was free of plant growth regulators, this medium was chosen for proliferation studies. The best rooting results were achieved in MS medium without auxins (92.00 ± 6.11%, 6.54 ± 0.52 and 1.60 ± 0.10 cm regarding rooting frequency, number of roots per shoot and longest roots, respectively) or supplemented with 0.5 mg l-1 indole-3-acetic acid (98.00 ± 2.11%, 11.14 ± 0.75 and 2.40 ± 0.24 cm, respectively). Plantlets were successfully acclimatised to ex vitro conditions with a survival rate of 93.33%. With the development of this micropropagation protocol, an important contribution has been made to the conservation of the endangered species T. lotocephalus.
- Impact of chitosan on plant tissue culture: recent applicationsPublication . Coelho, N.; Romano, A.Chitosan is a very versatile biomaterial with countless applications in different fields. Due to its biodegradability, non-toxicity, and biocompatibility, the use of chitosan is associated with sustainable practices. When applied to plants, in an agricultural context, chitosan induces defense mechanisms, stress resistance, and increased productivity and it has proven to be a feasible alternative to harmful agrochemicals. For in vitro plant culture, chitosan has been also applied to crops and other economically important species, with promising results. In this review, we discuss the recent applications of chitosan on plant tissue culture. Chitosan has been mainly used for the improvement of several growth parameters, but it has been also applied to enhance in vitro seed germination and accumulation of secondary metabolites. The plant material used, the concentrations and forms of chitosan employed, and the influence of chitosan on other plant parameters during in vitro culture are revised. We anticipate that chitosan applications will increase in a near future because of its potential and the increasing demand for more eco-friendly procedures.