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- Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markersPublication . Vergara-Chen, Carlos; González-Wangüemert, Mercedes; Bermingham, Eldredge; D’Croz, L.A molecular approach, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.
- A mitochondrial DNA based phylogeny of weakfish species of the Cynoscion group (Pisces: Sciaenidae)Publication . Vergara-Chen, Carlos; Aguirre, Windsor E.; González-Wangüemert, Mercedes; Bermingham, EldredgeWe infer the phylogeny of fishes in the New World Cynoscion group (Cynoscion, Isopisthus, Macrodon, Atractoscion, Plagioscion) using 1603bp of DNA sequence data from three mitochondrial genes. With the exception of Plagioscion, whose position was ambiguous, the Cynoscion group is monophyletic. However, several genera examined are not monophyletic. Atlantic and Pacific species of Cynoscion are interspersed in the tree and geminate species pairs are identified. Intergeneric relationships in the group are clarified. Our analysis is the first comprehensive phylogeny for the Cynoscion group based on molecular data and provides a baseline for future comparative studies of this important group.
- Molecular systematics of the genus Holothuria in the Mediterranean and Northeastern Atlantic and a molecular clock for the diversification of the Holothuriidae (Echinodermata: Holothuroidea)Publication . Borrero-Pérez, Giomar Helena; Gómez-Zurita, Jesús; González-Wangüemert, Mercedes; Marcos, Concepción; Pérez-Ruzafa, AngelThis work investigates the systematics of the genus Holothuria in the Mediterranean Sea and Northeastern Atlantic in the light of a wider molecular phylogenetic hypothesis of Holothuriidae, and it also provides a time-scale for the family diversification using mitochondrial markers and the molecular clock hypothesis. The subgenera Holothuria and Roweothuria are retrieved as paraphyletic. At least four separate lineages, with quite different time frameworks were identified. There are at least three species with an apparent long evolutionary history, H. forskali, H. sanctori and H. impatiens and six species belonging to Holothuria, Roweothuria and Vaneyothuria, which have diverged relatively recently.