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- Selective detection of human papilloma virus DNAs by specific synthetic DNA probesPublication . Cravador, A.; Herzog, A.; Houard, S.; D'ippolito, P.; Carroll, R.; Bollen, A.Specific oligodeoxynucleotide probes ranging from 20 to 35 nucleotides were defined to differentiate each of the HPV1a, 5, 6b, 8, 11, 16, 18 and 33. They were chosen using computer programs developed to compare simultaneously several 8000 bp long DNA sequences. Sequences common to all and to specific groups of the HPV DNA were also selected. Specificity of 32P-labelled probes for HPV6b, 11, 16, 18 and 33 was demonstrated and the sensitivity of the assays was evaluated by filter hybridization with viral clones and with DNA from cervical tumor biopsies. © 1989.
- A situação actual do Sida ao nível mundialPublication . Cravador, A.O objectivo deste artigo é informar sobre a situação actual do SIDA nos seus aspectos e implicações mais variados, da sua evolução, desde que foi descoberto e dos problemas sociais, políticos, legislativos e morais que o seu reconhecimento e a estratégia para o eliminar têm levantado em diferentes países.
- Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomiPublication . Coelho, A. C.; Cravador, A.; Bollen, A.; Ferraz, J. F. P.; Moreira, A. C.; Fauconnier, A.; Godfroid, EdmondIn response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identification method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-amplified target DNA. The target DNA is a 349 bp DNA fragment partially covering the 3'-translated and 3'- untranslated regions of the cinnamomin gene. When the specificity of the PCR reaction was evaluated in vitro using isolates of P. cinnamomi and eight other Phytophthora species, including the related P. cambivora, it was specific to P. cinnamomi. When 30 isolates of P. cinnamomi from oak roots in southern Portugal were assayed, 26 gave a strong positive response. The assay has a sensitivity of about 2±5 genome equivalents of P. cinnamomi. The reason for the negative response of four isolates remains unclear.
- Reduction vs acetalisation of some carbonyl-compounds by means of methylselenol and lewis-acidsPublication . Cravador, A.; Krief, A.; Hevesi, L.Aliphatic ketones and aromatic carbonyl compounds on reaction with methylselenol :and a Lewis acid, are shown to undergo acetalisation and/or reduction to methyl selenide, the unexpected reduction reaction being predominant in most cases.
- Nouvelles méthodes de synthèse utilisant une entité séléniée comme précurseur de fonctionPublication . Cravador, A.Nous sommes intéressés au cours des derniéres annés à la mise au point de schémas de synthèses utilisant une entité séléniée comme précurseur de fonction.
- Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferonsPublication . Wathelet, M.; Moutschen, S.; Defilippi, P.; Cravador, A.; Collet, M.; Huez, G.; Content, J.Among the various proteins which are induced when human cells are are treatened with interferon, a predominant protein of unknown function, with molecular mass 56 kDa, has been observed. With the aim of exploring the molecular basis of the regulation of this protein and of its mRNA, in order to understand its biological functionand its possible contribution to the various antiviral and non-antiviral actions exerted by interferons.
- Unexpectedly easy formation of alpha-seleno-carbonyl compounds from vinyl selenidesPublication . Cravador, A.; Krief, A.Seleno-carbonyl compounds are formed on reaction of seleninic acid or anhydride with vinyl selenides and vinyl sulphides.
- Cloning and expression in Escherichia coli of full-length complementary DNA coding for human α1-antitrypsinPublication . Bollen, A.; Herzog, A.; Cravador, A.; Hérion, P.; Chuchana, P.; Vander Straten, A.; Loriau, R.; Jacobs, P.; Van Elsen, A.A cDNA library prepared from human liver was screened for α₁-antitrypsin, a major constituent of plasma which functions as inhibitor of proteolytic enzyms. The library was screened using a 12-base-long synthetic oligodeoxyribonucleotide corresponding to a known DNA fragment of human α₁-antitrypsin and by hybrid-selection of α₁-antitrypsin mRNA.
- Unusual reactivity of selenoboranes towards epoxides: New selective routes to beta- hydroxyselenides and allylalcoholsPublication . Cravador, A.; Krief, A.Selenoboranes react with terminal, α, β-di- and trisubstituted epoxides to produce β-hydroxyselenides (or olefins) in the two first cases and allyl alcohols in the last one. A very high stereodescrimination has been observed for α, β-bisubstituted epoxides: the cis epoxide being much more reactive.
- Les oligonucléotides synthétiques antisens (2re partie)Publication . Cravador, A.La voie la plus utilisée pour synthétiser des conjugués d'oligodésoxyribonucléotides est la phosphorylation de l'extremité 5'OH avec des agents de phosphorylation appropriés.