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- A new species of Pontocrates Boeck, 1871 (Crustacea, Amphipoda, Oedicerotidae) from CyprusPublication . GARCIA GÓMEZ, SERGIO C.; MYERS, ALAN A.; AVRAMIDI, ELENI; GRAMMATIKI, KLEOPATRA; Lymperaki, Myrsini; RESAIKOS, VASILIS; PAPATHEODOULOU, MAGDALENE; LOUCA, VASILIS; XEVGENOS, DIMITRIOS; KÜPPER, FRITHJOF C.A new species of the amphipod genus Pontocrates (Boeck 1871), family Oedicerotidae, is described from Cyprus in the south-eastern Mediterranean Sea. It is a sister taxon to Pontocrates moorei (Myers & Ashelby 2022), currently recorded solely from the British Isles. It is the third species of Pontocrates now known to occur in the Mediterranean Sea.
- eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in CyprusPublication . Grammatiki, K.; Jonge, N. de; Nielsen, J. L.; García-Gomez, S. C.; Avramidi, E.; Lymperaki, Myrsini; Marcou, M.; Ioannou, G.; Papatheodoulou, M.; Dargent, O.; Xevgenos, D.; Hesselsøe, M.; Küpper, F. C.Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world - including Cyprus and the wider Mediterranean - Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.
- First record of the diatom pathogen Diatomophthora perforans cf. subsp. 2 pleurosigmae (Oomycota) from the Mediterranean microphytobenthosPublication . Scholz, Bettina; Grammatiki, Kleopatra; Avramidi, Eleni; Lymperaki, Myrsini; Resaikos, Vasilis; Papatheodoulou, Magdalene; Küpper, Frithjof C.Knowledge about pathogens of diatoms and macroalgae in the Mediterranean is scarce. This paper reports the first record of the oomycete Diatomophthora perforans subsp. pleurosigmae infecting the Mediterranean microphytobenthic diatom Pleurosigma cf. intermedium, which was detected in the context of marine environmental surveys of the brine outfalls of two seawater desalination plants.
- SAMMBA is a high-throughput pipeline for isolating and phenotyping macroalgal strainsPublication . Alves-Lima, Cicero; de Matos Barreto, Luís António; Monico, Carina; Gouvêa, Lidiane; Félix de Azeredo Pinto e Melo, Francisca; Varga, Brigitta; Filipe, Joana; Camacho, Rita; Lymperaki, Myrsini; Alberto, Filipe; Rörig, Leonardo R.; Engelen, Aschwin; Serrao, Ester A.; Pearson, Gareth Anthony; Martins, NeusaDespite successful preservation efforts, macroalgal diversity remains under-represented in global biobanks. A major limitation is the extreme morphological diversity of seaweed thalli, which hinders standardized isolation and phenotyping and often requires taxon-specific protocols. Here we present SAMMBA (Seaweed Automatable Microplate Microscopy for Breeding Approaches), an end-to-end pipeline for the high-throughput isolation, phenotyping and storage of macroalgal propagules in 384-well plates. By optimizing live-cell manipulation for chlorophyll autofluorescence (CAF) imaging and segmentation, multiple unialgal propagules can be isolated by dilution-based workflows. In a single plate, we obtained 68 singlet gametophyte fragments of Laminaria ochroleuca (17.7%) and 60 meiospores of Phyllariopsis purpurascens (31.25%). We demonstrated taxonomic and morphological versatility by isolating 60 unialgal cultures from three distinct Rhodophyta morphotypes (filamentous, crustose and foliose) and 10 strains of Ulva sp., also in a single plate. Furthermore, CAF-based area increase over 30 days enabled high-precision estimates of specific growth rates, yielding 0.130 ± 0.006 and 0.117 ± 0.01 day− 1 for male and female L. ochroleuca gametophytes, respectively (n = 768; p = 1.27e− 53). This precision substantially increases experimental reproducibility and statistical power compared to conventional methods, supporting high-throughput recovery of unialgal strains without motorized platforms, while remaining fully compatible with automation. SAMMBA expands operational capacity for strain discovery and phenotyping, providing a scalable foundation for phenomics, domestication workflows, and standardized macroalgal biobanking. We outline how the platform can benefit multiple areas of phycological research and facilitate the development of improved strains that can support aquaculture and restoration efforts.
- Environmental impact of brine from desalination plants on marine benthic diatom diversityPublication . Grammatiki, K.; Jonge, N. de; Nielsen, J. L.; Scholz, B.; Avramidi, E.; Lymperaki, Myrsini; Hesselsøe, M.; Xevgenos, Dimitris; Küpper, F. C.Benthic diatoms are sensitive indicators of environmental conditions at the seabed. In this study, benthic diatom communities at two brine outfall sites of reverse osmosis (RO) seawater desalination plants in Larnaca and Dhekelia, Cyprus, were investigated using a classical, microscopy-based approach and environmental DNA metabarcoding. In general, the diversity of diatoms measured by both methods (microscopy and eDNA metabarcoding), increased by distance from the brine discharge. Increased TOC and nutrient enrichment at brine outfalls contributed to decreased diatom diversity at the Larnaca outfalls, but the diatom diversity at Dhekelia was not driven by abiotic factors. The diatom communities at the outfalls were shown to be distinct and showed temporal variation across the sampling seasons with eDNA metabarcoding, but this was the case only for Dhekelia with microscopy. The results highlight the effect of local biogeography and different brine mixing methods on diatom diversity. The results revealed that conventional morphological methods and eDNA metabarcoding rarely leads to similar conclusions. However, the complementary results emphasise that more information can be derived when combining the methods for biodiversity impact assessments.