Loading...
2 results
Search Results
Now showing 1 - 2 of 2
- Brown seaweeds as a source of anti-hyaluronidase compoundsPublication . Arunkumar, K.; Raj, Reeshma; Rathinam, Raja; Carvalho, Isabel SHyaluronidase enzymes disrupt hyaluronic acid, causing angiogenesis, tumor invasiveness, metastasis, inflammation, and skin aging. Phlorotannin, alginate and fucoidan were extracted successively from the blade and stipe samples of two Sargassum seaweeds and Eisenia arborea thallus. These were evaluated for their in vitro anti-hyaluronidase and antioxidant activities (DPPH and reducing power assay). The crude phlorotannin content was highest in the blade of S. tenerrimum (22.405 +/- 3.6 mu g/mg), followed by S. vulgare blade (18.385 +/- 3.29 mu g/mg) with lower amounts in the stipe portion. The highest yield of alginate and fucoidans was obtained from the blade samples of S. vulgare (0.322 +/- 0.38 and 0.198 +/- 0.016%), followed by S. tenerrimum (0.090 +/- 0.01 and 0.063 +/- 0.005%) and E. arborea thallus (0.047 +/- 0.008 and 0.032 +/- 0.003%). The sulfate content was higher in fucoidan than alginate extracted from the stipe regions of the seaweeds. Phlorotannin, fucoidan and alginate from S. vulgare, S. tenerrimum, and E. arborea possessed anti-hyaluronidase activity as evident by a decrease in the N-acetylglucosamine release. The highest anti-hyaluronidase activity was achieved in the extract of S. tennerimum blade (37.67 +/- 2.3% inhibition) due to its high phlorotannin content. Alginate and fucoidan extracted from the stipes of Sargassum species possess higher bioactivities than the blade samples. The FTIR study ascertained that alginate with a high guluronic acid and high sulfated fucoidan were extracted from the stipe samples compared to the blade samples. This increased viscosity and promoted bioactivity respectively. Further studies to evaluate the emulsifying and viscosity properties of these compounds are required before they can be considered for commercial applications.
- Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweedsPublication . Arunkumar, K.; Rathinam, Raja; Kumar, V. B. Sameer; Joseph, Ashna; Shilpa, T.; Carvalho, Isabel S.In recent times, there has been a growing interest in the exploration of antioxidants and global trend toward the usage of seaweeds in the food industries. The low molecular weight up to 14 kDa sulfated polysaccharides of seaweeds (Portieria hornemannii, Spyridia hypnoides, Asparagopsis taxiformis, Centroceras clavulatum and Padina pavonica) were evaluated for in vitro antioxidant activities and cytotoxic assay using HeLa cell line and also characterized by FTIR. The high yield (7.74% alga dry wt.) of sulfated polysaccharide was observed in P. hornemannii followed by S. hypnoides (0.69%), C. clavulaum (0.55%) and A. taxiformis (0.17%). In the brown seaweed P. pavonica, the sulfated polysaccharide yield was 2.07%. High amount of sulfate was recorded in the polysaccharide of A. taxiformis followed by C. clavulaum, P. pavonica, S. hypnoides and P. hornemannii as indicative for bioactivity. The FTIR spectroscopic analysis supports the sulfated polysaccharides of S. hypnoides, C. clavulatum and A. taxiformis are similar to agar polymer whereas the spectral characteristics of P. hornemannii have similarities to carrageenan. The higher DPPH activity and reducing power were recorded in the polysaccharide of brown seaweed P. pavonica than the red seaweeds as follows: DPPH activities: S. hypnoides > A. taxiformis > C. clavulatum > P. hornimanii; Reducing power: A. taxiformis > P. hornimanii > S. hypnoides > C. clavulatum. The polysaccharide fractions contain up to 14 kDa from red seaweeds P. hornemannii and S. hypnoides followed by brown seaweed P. pavonica exhibit cytotoxic activity in HeLa cancer cell line (and are similar to structural properties of carrageenan extracted from P. hornemannii). The low molecular weight agar like polymer of S. hypnoides and alginate like brown seaweed P. pavonica showing better in vitro antioxidant activities that are capable of exhibiting cytotoxicity against HeLa cell line can be taken up further in-depth investigation for nutraceutical study.