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- A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markersPublication . Carlier, Jorge; Sousa, N. H.; Santo, Tatiana; d'Eeckenbrugge, G. C.; Leitão, JoséDespite the paramount importance of pineapple (Ananas comosus L.) in world production and trade of tropical fruits, the genomics of this crop is still lagging behind that of other tropical fruit crops such as banana or papaya. A genetic map of pineapple was constructed using an F2 segregating population obtained from a single selfed F1 plant of a cross A. comosus var. comosus (cv. Rondon, clone BR 50) x A. comosus var. bracteatus (Branco do mato, clone BR 20). Multiple randomly amplified markers (RAPD, ISSR and AFLP) were brought together with SSR and EST-SSR markers identified among sequences uploaded to public databases and with sequence-specific markers (SCAR, SSR and CAPS) derived from random amplified markers. Sixty-three randomly amplified markers (RAPD, ISSR and AFLP) were selected and cloned, resulting in 71 sequences which were used to generate sequence-specific SCAR and CAPS markers. The present map includes 492 DNA markers: 57 RAPD, 22 ISSR, 348 AFLP, 20 SSR, 12 EST-SSR, 25 SCARs, 8 CAPS, and the morphological trait locus "piping", gathered into 33 linkage groups that integrate markers inherited from both botanical varieties, four linkage groups with markers only from var. comosus and three linkage groups with markers exclusively from var. bracteatus. The relatively higher mapping efficiency of sequence-specific markers derived from randomly amplified markers (50.7%) versus SSR (31.4%) and EST-SSR (28.9%) markers is discussed. Spanning over 80% of the 2,470 cM estimated average length of the genome, the present map constitutes a useful research tool for molecular breeding and genomics projects in pineapple and other Bromeliaceae species.
- Genetic mapping of DNA markers in pineapplePublication . Carlier, Jorge; Nancheva, D.; Leitão, José; d'Eeckenbrugge, G. C.Two genetic maps of DNA-markers (RAPD, AFLP and ISSR) of Ananas comosus var. bracteatus and A. comosus var. comosus have been previously published by our team. These maps were constructed at the Laboratory of Genetics and Plant Breeding of FERN, University of Algarve, using an F1 mapping population derived from a cross performed in Martinique between the two botanical varieties. However, the use of a F1 population, the small size of this population and the use of molecular markers with dominant inheritance allowed only markers that were heterozygous in the parents to be included in those maps and prevented the construction of an integrated high-density map. A new genetic map, intended to cover all the pineapple genome, is currently under construction using a mapping population of 142 F2 plants. This new map will integrate the already published maps and will include markers that had remained unlinked, as they were polymorphic but homozygous in parental genotypes, as well as newly identified markers. At the present time, the new map consists of seven linkage groups (> 40 cM) that integrate markers from both parents, 21 groups (> 40 cM) with markers mostly from one parent, six groups (< 40 cM and > 25 cm) with markers from one parent, and 12 smaller (< 25 cM) linkage groups, covering approximately 62% of the pineapple genome.
- Genetic maps of RAPD, AFLP and ISSR markers in Ananas bracteatus and A-comosus using the pseudo-testcross strategyPublication . Carlier, Jorge; Reis, A.; Duval, M. F.; d'Eeckenbrugge, G. C.; Leitão, J. M.Genetic maps of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLP) and inter simple sequence repeats (ISSR) markers in pineapple (2n = 2x = 50) are reported for the first time. On the basis of a segregating population of 46 F1 individuals from a cross Ananas comosus x A. bracteatus, genetic maps of these two species were constructed using the two-way pseudo-testcross approach. The A. bracteatus map consists of 335 markers (60 RAPDs, 264 AFLPs and 11 ISSRs) assembled into 50 linkage groups, 26 of them with at least four markers. The A. comosus map consists of 157 markers (33 RAPDs, 115 AFLPs, eight ISSRs and the 'piping' trait locus) organized into 30 linkage groups, 18 of them with at least four markers. These maps cover, respectively, 57.2% of the A. bracteatus genome estimated as 3693 cM long, and 31.6% of the A. comosus genome calculated as 4146 cM. A rough estimate of 120 and 127 kbp/cM on average was found for the relationship between physical and genetic distance for A. bracteatus and A. comosus, respectively.