Loading...
2 results
Search Results
Now showing 1 - 2 of 2
- Bioelectrical signal detection using conducting polymer electrodes and the displacement current methodPublication . Inácio, Pedro; Mestre, Ana L G; Medeiros, C.R.; Asgarifar, Sanaz; ELAMINE, Youssef; Canudo, Joana; Santos, João; Bragança, José; Morgado, Jorge; Biscarini, Fabio; Gomes, Henrique L.Conducting polymer electrodes based on poly (3, 4 ethylenedioxythiophene): polystyrene sulfonate were used to record electrophysiological signals from autonomous cardiac contractile cells present in embryoid bodies. Signal detection was carried out by measuring the displacement current across the polymer/electrolyte double-layer capacitance, and compared with voltage detection. While for relatively low capacitance electrodes, the voltage amplification provides higher signal quality, and for high capacitive electrodes, the displacement current method exhibits a higher signal-to-noise ratio. It is proposed that the displacement current method combined with high capacitive polymer-based electrodes is adequate to measure clusters of cells and whole organs. Our approach has a great potential in fundamental studies of drug discovery and safety pharmacology.
- Extracellular bioelectrical lexicon: detecting rhythmic patterns within dermal fibroblast populationsPublication . Félix, Rute; Medeiros, Maria do Carmo; Elamine, Youssef; Power, Deborah Mary; Gomes, Henrique LeonelThis study uses a bioelectronic-based method to establish how non-electrogenic cells, like dermal fibroblast, employ bioelectrical signals to convey information. Electrophysiology using large-area Multielectrode Arrays (MEAs) devices revealed how populations of non-electrogenic cells in vitro generate patterns of bioelectrical signals. The period of the bioelectrical patterns depends on cell population activity. In a fully formed, healthy monolayer, bioelectrical activity is minimal. But during the formation of a monolayer, signals appear randomly, with a dominant period of 4.2 min. Occasionally, quasi-periodic bursts occur with a period between 1.6 and 2 min. When a mechanical wound is inflicted and during subsequent monolayer repair, quasi-periodic signal bursts occur, with an average period ranging from 60 to 110 min. The study uncovers a short-range non humoral communication system and a lexicon of bioelectrical signals linked to cell states.