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Research Project
Physiological bases for texture and color changes in fresh-cut ´Rocha´ pear and melon: implications on development of products and processes
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Volatile and quality changes in fresh-cut cantaloupe and honeydew melons stored in modified atmosphere packaging
Publication . Amaro, Ana L.; Beaulieu, John C.; Stein, Rebecca E.; Almeida, Domingos P. F.; Nunes, Carla
Modified atmosphere packaging (MAP) in fresh-cut melons has been reported to preserve the visual
quality during storage, yet its effect upon aroma and firmness is cultivar-dependent. The main objective
of this research was to compare the properties of fresh-cut from a fast senescing cantaloupe with a slow
senescing honeydew melon, regarding changes in quality and volatiles, when stored in passive MAP. Freshcut
cubes of cantaloupe and honeydew melons were packaged in polypropylene trays, over-wrapped with a
microperforated film and stored for 14 days at 5 ºC. Three replicate packages of each cultivar were assayed
at day 0, 4, 7, 11 and 14 for color, firmness, soluble solid content (SSC), respiration rate and volatile retention,
and the experiment was repeated. Volatile compounds were extracted using a relatively recent technique,
Stir Bar Sorptive Extraction (SBSE), and quantified via GC-MS. Color and SSC remained constant throughout
storage. During the first 4 days of storage, the cantaloupe cubes softened at a higher rate (2.0 N day-1) than
honeydew cubes (0.9 N day-1), but firmness remained relatively unchanged thereafter until the end of the
storage period. Fresh-cut cantaloupe cubes exhibited a higher respiration rate (17.5-43.4 mL CO2 kg-1 h-1)
than fresh-cut honeydew cubes (7.7–30.0 mL CO2 kg-1 h-1), throughout the entire storage period. Esters
and aldehydes were the major volatile compounds present in the samples of both cultivars, but cantaloupe
yielded higher concentrations of esters and aldehydes, and lower concentrations of alcohols than honeydew.
Esters increased their levels during storage, whereas alcohols and aldehydes decreased.
Physiological bases for texture and color changes in fresh-cut ‘rocha’ pear: implications for the development of products and processes
Publication . Gomes, Maria Helena; Almeida, Domingos P. F.; Nunes, Carla
The physiological bases for the effectiveness of technologies used in fresh-cut fruit were examined in an
attempt to foster the shift from a market-pull to a science-push innovation model. Respiration rate of
fresh-cut ‘Rocha’ pear were measured at various oxygen concentrations. Apparent Km values for oxygen
uptake were very close to the fermentation thresholds, indicating that it is physiologically impossible to
reduce the respiration rate of fresh-cut pear by more than 50% without inducing fermentation. Based
on this information, we hypothesize that optimizing oxygen concentration inside packages is of little or
no value in fresh-cut pears. Additionally, additives used to reduce enzymic browning and softening may
have pleiotropic effects on quality. It was found that pH significantly affected the rate and intensity of
browning of fresh-cut pears. Browning was more intense in pears treated at pH 3.0 and less pronounced
at pH 7.0, but the softening rate was lower at the latter pH value. Calcium salts also affected texture and
color in a salt-specific manner. Larger color changes were observed with propionate and lactate. Although
calcium ascorbate reduced color changes, fruit slices treated with this salt were softer than those treated
with calcium propionate, lactate or chloride. Based on the physiological information gathered, we will
develop and evaluate an integrated process to maximize the quality of fresh-cut ‘Rocha’ pear throughout
the distribution chain.
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
3599-PPCDT
Funding Award Number
PTDC/AGR-ALI/66144/2006