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Study of modes of action of the biocontrol agent metschnikowia and auensis pbc-2
Publication . Manso, Teresa; Vero, Silvana; González, M. Belén; Nunes, Carla; Nunes, Carla
Metschnikowia andauensis NCYC 3728 (PBC-2) is an effective antagonist against the postharvest pathogens
Penicillium expansum, Botrytis cinerea and Rhizopus stolofiner on pome fruits, however its mode of action is
unknown. The ability of this strain to produce inhibitory compounds in 4 distinct media (PDA, NYDA, YPDA,
CJA) at 3 temperatures (1, 25, 30 ºC), was investigated. It was also assayed the competition for iron in media
with different iron concentrations and characterized the capability of PBC-2 produce and secrete fungal cell wall
lytic enzymes, like chitinase, protease, and glucanase in a culture media with fungal pathogen cell wall as unique
carbon source. M. andauensis PBC-2 did not show any inhibition zone to cope pathogens in any of the tested
media. The results obtained in this study suggest that the production and secretion of lytic enzymes is not the
main or more important mode of action of the new biocontrol agent PBC-2, since the production of chitinase
was observed only past 5 and 7 d of incubation, and the production of -1.3-glucanases and proteases was not
observed, which mean that the biocontrol agent PBC-2 have more than one mechanism of action.
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Fundação para a Ciência e a Tecnologia
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SFRH
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SFRH/BD/21922/2005