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Yeast extract elicitation enhances growth and metabolite production in Limonium algarvense callus cultures

2025-02Journal article Open access
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dc.contributor.authorLescano, Leonardo
dc.contributor.authorCziáky, Zoltán
dc.contributor.authorCustódio, Luísa
dc.contributor.authorRodrigues, Maria João
dc.date.accessioned2025-03-07T10:40:35Z
dc.date.available2025-03-07T10:40:35Z
dc.date.issued2025-02
dc.description.abstractLimonium algarvense Erben, a medicinal halophyte, holds significant pharmacological promise due to its rich bioactive compound repertoire. This study aimed to establish robust callus cultures as a sustainable, in vitro model for studying the plant's metabolic responses, particularly focusing on synthesising and accumulating primary and secondary metabolites under various elicitation treatments. Callus cultures were initiated from leaf explants on Murashige and Skoog's medium supplemented with 1 mg/L picloram for 4 weeks. Afterwards, callus cultures were subjected to two elicitor treatments, including salicylic acid-SA and yeast extract-YE at 50 and 100 mg/L for four weeks. Water extracts were assessed for their shifts in primary (total soluble sugars and proteins, and proline), and secondary metabolism (total phenolics, flavonoids, and condensed tannins). In addition, a detailed metabolic profiling was conducted using high-performance liquid chromatography with electrospray ionisation mass spectrometry (HPLC-ESI-MS/MS). Elicitation induced significant shifts in the metabolite synthesis of elicited cultures compared to controls. While YE50 markedly increased the callus yield, the total levels of phenolics, flavonoids condensed tannins and total soluble proteins, the SA50 led to the highest increase in proline content. Metabolomic analysis identified 10 metabolites, including 4-hydroxyphenyllactic acid, hydroxybenzoic acid, riboflavin (Vitamin B2), and dihydroferulic acid methyl ester 4-O-sulfate, that were increased in the YE50 elicitation treatment. This suggests that elicitation can effectively enhance the biosynthesis of primary and secondary metabolites in L. algarvense callus cultures, offering great potential for nutritional and medicinal applications.eng
dc.identifier.doi10.1007/s11240-025-02991-x
dc.identifier.eissn1573-5044
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/10400.1/26869
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofPlant Cell, Tissue and Organ Culture (PCTOC)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSea lavender
dc.subjectSalt-tolerant plant
dc.subjectHalophyte
dc.subjectPlant tissue culture
dc.titleYeast extract elicitation enhances growth and metabolite production in Limonium algarvense callus cultureseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue2
oaire.citation.startPage45
oaire.citation.titlePlant Cell, Tissue and Organ Culture
oaire.citation.volume160
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCustódio
person.familyNameRodrigues
person.givenNameLuísa
person.givenNameMaria João
person.identifierR-004-VNG
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0001-8732-710X
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56031608100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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