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Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

2001Journal article Open access
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dc.contributor.authorMoutou, K. A.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorMamuris, Z.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-15T10:18:45Z
dc.date.available2014-05-15T10:18:45Z
dc.date.issued2001
dc.date.updated2014-05-13T10:28:43Z
dc.description.abstractTwo full-length cDNA clones encoding the skeletal myosin light chain 2 (MLC2; 1452 bp) and myosin light chain 3 (MLC3; 972 bp) were isolated from a cDNA library prepared from gilthead sea bream Sparus aurata larvae. The MLC2 cDNA encoded a predicted protein of 170 residues that was 79 % identical to rabbit MLC2 over the entire length and 87 % identical within the Ca2+- binding region. The deduced amino acid sequence of MLC3 was 153 residues in length and was 91 % and 69% identical to the zebrafish and rabbit MLC3, respectively. Northern blot analysis revealed that in adults both transcripts were expressed in fast white muscle only. MLC2 appeared earlier in development: MLC2 transcripts were detectable from the beginning of segmentation, whereas MLC3 transcripts did not appear until 27 h post-fertilisation. At this developmental stage, a second MLC2 transcript of 0.89 kilobase-pairs was present. MLCs exhibited a different age-related pattern of response to varied thyroidal states, which were experimentally induced by the administration of 1 mgg-1 body mass of thyroxine (T4) or triiodothyronine (T3), or 5 ng g-1 body mass of the hypothyroidal compound thiourea; MLC3 expression was not significantly affected, whereas levels of MLC2 transcripts were significantly elevated in the white muscle only of juvenile sea bream after administration of T4. Although the mechanism of thyroidal regulation of MLC expression remains unknown, the present results suggest that different regulatory mechanisms exist for different MLCs.por
dc.identifier.citationMoutou, K. A.; Canario, A. V. M.; Mamuris, Z.; Power, D. M. Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation, Journal of Experimental Biology, 204, 17, 3009-3018, 2001.por
dc.identifier.issn0022-0949
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3950
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCompany of Biologistspor
dc.subjectThyroid regulationpor
dc.subjectDevelopmentpor
dc.subjectSea breampor
dc.subjectSparus auratapor
dc.subjectMyosin light chainpor
dc.subjectWhite musclepor
dc.titleMolecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3018por
oaire.citation.issue17por
oaire.citation.startPage3009por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume204por
person.familyNameCanario
person.familyNamePower
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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