2.P3. Cloning and gene expression of two novel stanniocalcin genes in the puffer fish Tetraodon nigroviridis

Schein,  V; Schein, Vanessa; Pinto, Patricia IS; Canario, Adelino V. M.

http://hdl.handle.net/10400.1/3967

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Authors

Schein, V; Schein, Vanessa

Pinto, Patricia IS

Canario, Adelino V. M.

Abstract(s)

Stanniocalcin 1 (STC1) is a hypocalcemic hormone first described in fishes. Recently a second gene (STC2) was isolated from mammals and fishes. However, in silico analysis of Tetraodon nigroviridis and Takifugu rubripes genomes revealed the presence of a third and a fourth gene. In this study STC1 was cloned from de Corpuscles of Stannius (CS), STC2 from the muscle, and the two novel genes identified, STC1B and STC2B from the brain of Tetraodon. The STC-1B gene encodes an ORF of 287aa and contains 11 conserved cyteine residues and the Nlinked glycosylation site, present in all identified STC1.

URI

http://hdl.handle.net/10400.1/3967

Citation

Schein, V.; Pinto, P.; Canário, A.V.M. 2.P3. Cloning and gene expression of two novel stanniocalcin genes in the puffer fish Tetraodon nigroviridis, Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 148, Supp, 10-10, 2007.