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Biocompatibility and stability of polysaccharide polyelectrolyte complexes aimed at respiratory delivery

2015Journal article Open access
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dc.contributor.authorRodrigues, Susana
dc.contributor.authorCardoso, Lurdes
dc.contributor.authorCosta, Ana M. Rosa da
dc.contributor.authorGrenha, Ana
dc.date.accessioned2016-01-06T17:13:02Z
dc.date.available2016-01-06T17:13:02Z
dc.date.issued2015
dc.description.abstractChitosan (CS) and chondroitin sulfate (CHS) are natural polymers with demonstrated applicability in drug delivery, while nanoparticles are one of the most explored carriers for transmucosal delivery of biopharmaceuticals. In this work we have prepared CS/CHS nanoparticles and associated for the first time the therapeutic protein insulin. Fluorescein isothiocyanate bovine serum albumin (FITC-BSA) was also used to enable comparison of behaviors regarding differences in molecular weight (5.7 kDa versus 67 kDa). Nanoparticles of approximately 200 nm and positive zeta potential around +20 mV were obtained. These parameters remained stable for up to 1 month at 4 C. Proteins were associated with efficiencies of more than 50%. The release of FITC-BSA in PBS pH 7.4 was more sustained (50% in 24 h) than that of insulin (85% in 24 h). The biocompatibility of nanoparticles was tested in Calu-3 and A549 cells by means of three different assays. The metabolic assay MTT, the determination of lactate dehydrogenase release, and the quantification of the inflammatory response generated by cell exposure to nanoparticles have indicated an absence of overt toxicity. Overall, the results suggest good indications on the application of CS/CHS nanoparticles in respiratory transmucosal protein delivery, but the set of assays should be widened to clarify obtained results.pt_PT
dc.identifier.citationMaterials 2015, 8, 5647-5670pt_PT
dc.identifier.doihttp://dx.doi.org/10.3390/ma8095268pt_PT
dc.identifier.issn1996-1944
dc.identifier.otherAUT: AMG02212; AMC01695;
dc.identifier.urihttp://hdl.handle.net/10400.1/7391
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
dc.relationDEVELOPMENT OF POLYSACCHARIDE-BASED CARRIERS FOR PULMONARY TUBERCULOSIS THERAPY
dc.relation.publisherversionhttp://www.mdpi.com/1996-1944/8/9/5268/htmlpt_PT
dc.subjectBiocompatibilitypt_PT
dc.subjectChitosanpt_PT
dc.subjectChondroitin sulfatept_PT
dc.subjectNasal deliverypt_PT
dc.subjectPolyelectrolyte complexespt_PT
dc.subjectPolysaccharidespt_PT
dc.subjectProtein deliverypt_PT
dc.subjectPulmonary deliverypt_PT
dc.titleBiocompatibility and stability of polysaccharide polyelectrolyte complexes aimed at respiratory deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
oaire.awardTitleDEVELOPMENT OF POLYSACCHARIDE-BASED CARRIERS FOR PULMONARY TUBERCULOSIS THERAPY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-FCF%2F100291%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0094%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FQUI%2FUI4023%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F52426%2F2013/PT
oaire.citation.endPage5670pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage5647pt_PT
oaire.citation.titleMaterialspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
person.familyNameRodrigues
person.familyNameCARDOSO
person.familyNameRosa da Costa
person.familyNameGrenha
person.givenNameSusana
person.givenNameLURDES
person.givenNameAna M
person.givenNameAna
person.identifier305029
person.identifier.ciencia-id9D1A-6537-0383
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-0329-4265
person.identifier.orcid0000-0002-1142-7689
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridE-2165-2012
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id55125292100
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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