dc.contributor.author	Ebadzad, G.
dc.contributor.author	Cravador, A.
dc.date.accessioned	2014-11-13T15:00:48Z
dc.date.available	2014-11-13T15:00:48Z
dc.date.issued	2014-10-17
dc.date.updated	2014-11-03T20:02:55Z
dc.description.abstract	cDNA-AFLP methodology was used to gain insight into gene fragments differentially present in the mRNA profiles of Quercus suber roots infected with zoospores of Phytophthora cinnamomi at different post challenge time points. Fifty-three transcript-derived fragments (TDFs) were identified and sequenced. Six candidate genes were selected based on their expression patterns and homology to genes known to play a role in defence. They encode a cinnamyl alcohol dehydrogenase2 (QsCAD2), a protein disulphide isomerase (QsPDI), a CC-NBS-LRR resistance protein (QsRPc), a thaumatin-like protein (QsTLP), a chitinase (QsCHI) and a 1,3-β-glucanase (QsGlu). Evaluation of the expression of these genes by quantitative polymerase chain reaction (qPCR) revealed that transcript levels of QsRPc, QsCHI, QsCAD2 and QsPDI increased during the first 24 h post-inoculation, while those of thaumatin-like protein decreased. No differential expression was observed for 1,3-β-glucanase (QsGlu).Four candidate reference genes, polymerase II (QsRPII), eukaryotic translation initiation factor 5A (QsEIF-5A), β-tubulin (QsTUB) and a medium subunit family protein of clathrin adaptor complexes (QsCACs) were assessed to determine the most stable internal references for qRT-PCR normalization in the Phytophthora-Q. suber pathosystem in root tissues. Those found to be more stable, QsRPII and QsCACs, were used as internal reference in the present work.Knowledge on the Quercus defence mechanisms against biotic stress is scarce. This study provides an insight into the gene profiling of a few important genes of Q. suber in response to P. cinnamomi infection contributing to the knowledge of the molecular interactions involving Quercus and root pathogens that can be useful in the future to understand the mechanisms underlying oak resistance to soil-borne oomycetes.	por
dc.description.version	Peer Reviewed
dc.identifier.citation	SpringerPlus. 2014 Oct 17;3(1):613	por
dc.identifier.doi	http://dx.doi.org/10.1186/2193-1801-3-613
dc.identifier.other	AUT: ACR00659;
dc.identifier.uri	http://hdl.handle.net/10400.1/5625
dc.language.iso	eng
dc.peerreviewed	yes	por
dc.publisher	SpringerPlus	por
dc.relation	Morphological Impacts and COastal Risks induced by Extreme storm events
dc.rights.holder	Ghazal Ebadzad et al.; licensee BioMed Central Ltd.
dc.subject	Cork oak	por
dc.subject	Reference genes	por
dc.subject	Defence response	por
dc.subject	qRT-PCR	por
dc.subject	Biotic stress	por
dc.title	Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection	por
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	Morphological Impacts and COastal Risks induced by Extreme storm events
oaire.awardURI	info:eu-repo/grantAgreement/EC/FP7/202798
oaire.awardURI	info:eu-repo/grantAgreement/EC/FP7/202798/EU
oaire.awardURI	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F76979%2F2011/PT
oaire.citation.endPage	13	por
oaire.citation.issue	(1):613	por
oaire.citation.startPage	2	por
oaire.citation.title	SpringerPlus	por
oaire.citation.volume	3	por
oaire.fundingStream	FP7
oaire.fundingStream	FP7
oaire.fundingStream	SFRH
person.familyName	Cravador
person.givenName	Alfredo
person.identifier.orcid	0000-0002-9831-9815
person.identifier.rid	K-7247-2012
person.identifier.scopus-author-id	6602537257
project.funder.identifier	http://doi.org/10.13039/501100008530
project.funder.identifier	http://doi.org/10.13039/501100008530
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	European Commission
project.funder.name	European Commission
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery	8c84a9e6-6b7f-4b50-93a2-152c85901722
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Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection

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