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Antioxidant systems and lipid peroxidation in from Mid-Atlantic Ridge hydrothermal vent fields

2005Journal article Restricted access
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dc.contributor.authorBebianno, Maria João
dc.contributor.authorCompany, Rui
dc.contributor.authorSerafim, Angela
dc.contributor.authorCamus, L.
dc.contributor.authorCosson, R.
dc.contributor.authorFialamedoni, A.
dc.date.accessioned2020-05-08T09:02:26Z
dc.date.available2020-05-08T09:02:26Z
dc.date.issued2005
dc.description.abstractEnzymatic defenses involved in protection from oxygen radical damage were determined in gills and mantle of Bathymodiolus azoricus collected from three contrasting Mid-Atlantic Ridge (MAR) hydrothermal vent fields (Menez-Gwen, Lucky Strike and Rainbow). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx) (total and Se-dependent), and levels of total oxyradical scavenging capacity (TOSC), metallothioneins (MT) and lipid peroxidation (LPO) were determined in B. azoricus tissues and the impact of metal concentrations on these antioxidant systems and lipid peroxidation assessed. SOD, CAT, TOSC, MTs and LPO levels were higher in B. azoricus gills while glutathione peroxidases (total and Se-dependent) were higher in the mantle, and with the exception of CAT, were of the same order of magnitude as in other molluscs. TOSC levels from Menez-Gwen indicate that the vent environment at this site is less stressful and the formation of ROS in mussels is effectively counteracted by the antioxidant defense system. TOSC depletion indicates an elevated ROS production in molluscs at the other two vent sites. Cytosolic SOD, GPx and LPO were more relevant at Lucky Strike (Bairro Alto) where levels of essential (Cu and Zn) and toxic metals (Cd and Ag) were highest in the organisms. CAT activity and LPO were predominant at the Rainbow vent site, where an excess of Fe in mussel tissues and in vent fluids (the highest of all three vent sites) may have contributed to increased LPO. Therefore, three distinct pathways for antioxidant enzyme systems and LPO based on environmental metal speciation of MAR vent fields are proposed for Bathymodiolus gills. At Menez-Gwen, TOSC towards peroxyl and hydroxyl radicals and peroxynitrite are predominant, while at Lucky Strike cytosolic SOD activity and GPx are the main antioxidant mechanisms. Finally at Rainbow, catalase and lipid peroxidation are dominant, suggesting that resistance of mussels to metal toxicity at these vent fields decreases in the sequence Menez-Gwen > Lucky Strike and Rainbow.pt_PT
dc.description.sponsorshipATOS cruise and D. Dixon, VENTOX (EVK3 CT1999-00003) project coordinator and the crew of N/O Atalante and Victor 6000 (IFREER); “Marine Resource, Environment and Management”, project no. 146478/120 and by the Norwegian marine research laboratory RFAkvamiljø.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2005.08.013pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/13855
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnalysis of variancept_PT
dc.subjectAnimalspt_PT
dc.subjectAtlantic Oceanpt_PT
dc.subjectBivalviapt_PT
dc.subjectCatalasept_PT
dc.subjectFree radical scavengerspt_PT
dc.subjectGeographypt_PT
dc.subjectGillspt_PT
dc.subjectGlutathione peroxidasept_PT
dc.subjectLipid peroxidationpt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectHeavy metalspt_PT
dc.subjectPrincipal component analysispt_PT
dc.subjectSeawaterpt_PT
dc.subjectSuperoxide dismutasept_PT
dc.subjectAntioxidant systemspt_PT
dc.subjectHydrothermal vent fieldpt_PT
dc.subjectBathymodiolus azoricuspt_PT
dc.subjectTOSCpt_PT
dc.subjectMetallothioneinspt_PT
dc.titleAntioxidant systems and lipid peroxidation in from Mid-Atlantic Ridge hydrothermal vent fieldspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F904%2F2000/PT
oaire.citation.endPage373pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage354pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume75pt_PT
oaire.fundingStreamSFRH
person.familyNameBebianno
person.familyNamePereira Serafim
person.givenNameMaria
person.givenNameMaria Angela
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.ciencia-id9C18-090A-5E3D
person.identifier.orcid0000-0003-1492-8566
person.identifier.orcid0000-0002-1165-0645
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isAuthorOfPublication.latestForDiscovery0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isProjectOfPublication37fba9b6-0849-4b99-a48e-745a4b26e419
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