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Molecular effects of Microcystin-LA in tilapia (Oreochromis niloticus)

dc.contributor.authorMoreira, Daniel A.
dc.contributor.authorSoares, Raquel M.
dc.contributor.authorValente, Richard H.
dc.contributor.authorBebianno, Maria João
dc.contributor.authorRebelo, Mauro F.
dc.date.accessioned2020-04-03T15:36:18Z
dc.date.available2020-04-03T15:36:18Z
dc.date.issued2019
dc.description.abstractNile tilapia (Oreochromis niloticus) is a freshwater phytoplanktivorous fish species reported to accumulate and tolerate large amounts of cyanotoxins such as microcystins (MCs). The present study aimed to investigate molecular responses to the acute exposure of Nile tilapia to the Microcystin-LA analogue (MC-LA). Thus, the specimens were sublethally exposed to 1000 μg kg-1 of MC-LA for 12, 24, 48, and 96 h. Gene expression of PP1, PP2A, GST, GPX and actin was analyzed by quantitative PCR. The protein abundance profile of PP2A was determined by immunoblotting, while the integrity of its biological function was assessed by a phosphatase enzymatic assay. PP2A activity was significantly and strongly reduced by MC-LA. A resulting feedback mechanism significantly increased PP2A gene expression and protein abundance in all assessed times. However, a recovery of that phosphatase activity was not observed. In this study, the observed increase in GPX gene expression was the only response that could be directly related to the unknown factors associated to the fish survival to such high dose exposure.pt_PT
dc.description.sponsorshipE-26/111.540/2008pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.toxicon.2019.05.002pt_PT
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10400.1/13688
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0041010119301461?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectActinspt_PT
dc.subjectAnimalspt_PT
dc.subjectCichlidspt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectGlutathione peroxidasept_PT
dc.subjectGlutathione transferasept_PT
dc.subjectInjectionspt_PT
dc.subjectMicrocystinspt_PT
dc.subjectProtein phosphatase 1pt_PT
dc.subjectProtein phosphatase 2pt_PT
dc.subjectIntraperitonealpt_PT
dc.titleMolecular effects of Microcystin-LA in tilapia (Oreochromis niloticus)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage82pt_PT
oaire.citation.startPage76pt_PT
oaire.citation.titleToxiconpt_PT
oaire.citation.volume166pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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