Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

Pereira, Hugo; Barreira, Luísa; Mozes, Andre; Florindo, C.; Polo, Cristina; Duarte, Catarina V.; Custódio, Luísa; Varela, J.

http://hdl.handle.net/10400.1/917

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Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.