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Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

2011-12-22Journal article Open access
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dc.contributor.authorPereira, Hugo
dc.contributor.authorBarreira, Luísa
dc.contributor.authorMozes, Andre
dc.contributor.authorFlorindo, C.
dc.contributor.authorPolo, Cristina
dc.contributor.authorDuarte, Catarina V.
dc.contributor.authorCustódio, Luísa
dc.contributor.authorVarela, J.
dc.date.accessioned2012-02-29T23:36:36Z
dc.date.available2012-02-29T23:36:36Z
dc.date.issued2011-12-22
dc.date.updated2012-02-27T16:07:00Z
dc.description.abstractAbstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.por
dc.description.versionPeer Reviewed
dc.identifier.citationBiotechnology for Biofuels. 2011 Dec 22;4(1):61por
dc.identifier.doihttp://dx.doi.org/10.1186/1754-6834-4-61
dc.identifier.otherAUT: LBA00541; JVA01305;
dc.identifier.urihttp://hdl.handle.net/10400.1/917
dc.language.isoengpor
dc.language.rfc3066eng
dc.peerreviewedyespor
dc.relationSCREENING FOR BIOLOGICAL ACTIVITIES FROM MARINE PHOTOSYNTHETIC ORGANISMS FROM THE ALGARVE COAST
dc.relationMarine photosynthetic organisms of the Algarve coast with biomedical applications
dc.rights.holderPereira et al.; licensee BioMed Central Ltd.
dc.titleMicroplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgaepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSCREENING FOR BIOLOGICAL ACTIVITIES FROM MARINE PHOTOSYNTHETIC ORGANISMS FROM THE ALGARVE COAST
oaire.awardTitleMarine photosynthetic organisms of the Algarve coast with biomedical applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F65116%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F103957%2F2008/PT
oaire.fundingStream3599-PPCDT
person.familyNamePereira
person.familyNameBarreira
person.familyNameCustódio
person.familyNameVarela
person.givenNameHugo
person.givenNameLuísa
person.givenNameLuísa
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-6101-2013
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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