A carregar...
Publicação
Exploring Omega-3′s impact on the expression of Bone-Related Genes in Meagre (Argyrosomus regius)
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.32 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Dietary supplementation with Omega-3 fatty acids seems to promote skeletal health. Therefore, their consumption at imbalanced or excessive levels has offered less beneficial or even prejudicial
effects. Fish produced in aquaculture regimes are prone to develop abnormal skeletons. Although
larval cultures are usually fed with diets supplemented with Omega-3 Long Chain Polyunsaturated
fatty acids (LC-PUFAs), the lack of knowledge about the optimal requirements for fatty acids or about
their impact on mechanisms that regulate skeletal development has impeded the design of diets that
could improve bone formation during larval stages when the majority of skeletal anomalies appear. In
this study, Argyrosomus regius larvae were fed different levels of Omega-3s (2.6% and 3.6% DW on diet)
compared to a commercial diet. At 28 days after hatching (DAH), their transcriptomes were analyzed
to study the modulation exerted in gene expression dynamics during larval development and identify
impacted genes that can contribute to skeletal formation. Mainly, both levels of supplementation
modulated bone-cell proliferation, the synthesis of bone components such as the extracellular matrix,
and molecules involved in the interaction and signaling between bone components or in important
cellular processes. The 2.6% level impacted several genes related to cartilage development, denoting
a special impact on endochondral ossification, delaying this process. However, the 3.6% level seemed
to accelerate this process by enhancing skeletal development. These results offered important insights
into the impact of dietary Omega-3 LC-PUFAs on genes involved in the main molecular mechanism
and cellular processes involved in skeletal development.
Descrição
Palavras-chave
Fish larvae Skeletal development Differentially expressed genes Cartilage
Contexto Educativo
Citação
Biomolecules 14 (1): 56 (2024)
Editora
MDPI