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Heterotrophic and photoautotrophic media optimization using response surface methodology for the Novel Microalga Chlorococcum amblystomatis

2023-02-06Journal article Open access
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dc.contributor.authorCorreia, Nádia
dc.contributor.authorPereira, Hugo
dc.contributor.authorSchulze, Peter S.C.
dc.contributor.authorCosta, Monya
dc.contributor.authorSanto, Gonçalo E.
dc.contributor.authorGuerra, Inês
dc.contributor.authorTrovão dos Santos, Mafalda
dc.contributor.authorBarros, Ana
dc.contributor.authorCardoso, Helena
dc.contributor.authorSilva, Joana L.
dc.contributor.authorGouveia, Luisa
dc.contributor.authorVarela, João
dc.date.accessioned2023-02-28T11:10:29Z
dc.date.available2023-02-28T11:10:29Z
dc.date.issued2023-02-06
dc.date.updated2023-02-24T14:09:06Z
dc.description.abstractThe nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass composition and their biotechnological potential. Media optimization through surface response methodology led to 44.9 and 51.2% increments in C. amblystomatis-specific growth rates under heterotrophic and photoautotrophic growth, respectively. This microalga registered high protein content (61.49–73.45% dry weight), with the highest value being observed in the optimized photoautotrophic growth medium. The lipid fraction mainly constituted polyunsaturated fatty acids, ranging from 44.47 to 51.41% for total fatty acids (TFA) in cells under heterotrophy. However, these contents became significantly higher (70.46–72.82% TFA) in cultures cultivated under photoautotrophy. An interesting carotenoids content was achieved in the cultures grown in optimized photoautotrophic medium: 5.84 mg·g−1 β-carotene, 5.27 mg·g−1 lutein, 3.66 mg·g−1 neoxanthin, and 0.75 mg·g−1 violaxanthin. Therefore, C. amblystomatis demonstrated an interesting growth performance and nutritional profile for food supplements and feed products that might contribute to meeting the world’s nutritional demand.pt_PT
dc.description.sponsorshipLISBOA-01-0247-FEDER-035234
dc.description.sponsorshipLA/P/0101/2020
dc.description.sponsorshipPOCI-01-0247-FEDER-035234
dc.description.sponsorship023310–ALGACO2
dc.description.sponsorshipALG-01-0247-FEDER-035234
dc.description.sponsorshipALG-01-0247-FEDER-069961
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationApplied Sciences 13 (4): 2089 (2023)pt_PT
dc.identifier.doi10.3390/app13042089pt_PT
dc.identifier.eissn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.1/19153
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicroalgaept_PT
dc.subjectChlorococcum amblystomatispt_PT
dc.subjectGrowth optimizationpt_PT
dc.subjectResponse surface methodologypt_PT
dc.subjectHetero- and photoautotrophic cultivationpt_PT
dc.titleHeterotrophic and photoautotrophic media optimization using response surface methodology for the Novel Microalga Chlorococcum amblystomatispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue4pt_PT
oaire.citation.startPage2089pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCorreia
person.familyNamePereira
person.familyNameSchulze
person.familyNameCosta
person.familyNameTrovão dos Santos
person.familyNameGouveia
person.familyNameVarela
person.givenNameNádia
person.givenNameHugo
person.givenNamePeter S.C.
person.givenNameMonya
person.givenNameMafalda
person.givenNameLuisa
person.givenNameJoão
person.identifier282781
person.identifierH-4046-2011
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person.identifier.ciencia-idF41E-3B55-577C
person.identifier.ciencia-idD912-559A-7706
person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0001-5411-8686
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3777-8732
person.identifier.orcid0000-0002-4236-9063
person.identifier.orcid0000-0002-9465-7681
person.identifier.orcid0000-0003-0196-6360
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id56244247100
person.identifier.scopus-author-id8094380800
person.identifier.scopus-author-id7004135834
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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