Repository logo
 
Publication

Comparing p20's RNA silencing suppressing activity among five phylogenetic groups of Citrus Tristeza virus

dc.contributor.authorMarques, N T.
dc.contributor.authorCosta, A. A.
dc.contributor.authorLopes, D.
dc.contributor.authorSilva, Goncalo
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2015-06-15T14:13:46Z
dc.date.available2015-06-15T14:13:46Z
dc.date.issued2012
dc.description.abstractThe p20 protein encoded by the Citrus Tristeza Virus (CTV) was previously identified as a RNA silencing suppressor. In this study, we analyzed the p20's suppressing activity from five phylogenetic groups of CTV, using the co-infiltration assay of Green fluorescence protein (GFP) gene and the suppressor gene in 16C line Nicotiana benthamiana plants. Green fluorescence, GFP mRNA relative levels and GFP specific siRNAS were compared showing in most cases, only slight differences. Contrary to previous studies, the p20 suppressor was not able to impede neither short range nor systemic spreading of RNA silencing. The suppressor from the phylogenetic group 4 revealed a much reduced activity when compared with the others. At present we still don't know whether this property is a characteristic of this group or an atypical feature due to a unique point mutation. The differences in the symptom type and intensity originated by isolates belonging to the phylogenetic groups assayed could not be related to differences to the p20 suppressor's activity.
dc.identifier.doihttps://dx.doi.org/10.1007/s10658-011-9877-0
dc.identifier.issn0929-1873
dc.identifier.otherAUT: GNO00324; NMA00640;
dc.identifier.urihttp://hdl.handle.net/10400.1/6236
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-002-ARQ
dc.titleComparing p20's RNA silencing suppressing activity among five phylogenetic groups of Citrus Tristeza virus
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage235
oaire.citation.startPage229
oaire.citation.titleEuropean Journal of Plant Pathology
oaire.citation.volume133
person.familyNameTomás Marques
person.familyNameSilva
person.familyNameNolasco
person.givenNameNatália
person.givenNameGoncalo
person.givenNameGustavo
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.orcid0000-0002-0222-621X
person.identifier.orcid0000-0001-5544-2947
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublication0e6c622a-5843-4e5c-bc68-96c9206cb452
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery0e6c622a-5843-4e5c-bc68-96c9206cb452

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Comparing p20’s RNA silencing suppressing activity among five phylogenetic groups of Citrus Tristeza virus.pdf
Size:
397.73 KB
Format:
Adobe Portable Document Format