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Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production

2021-06Journal article Restricted access
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dc.contributor.authorConstantino, Ana
dc.contributor.authorRodrigues, Brígida
dc.contributor.authorLeon, R.
dc.contributor.authorBarros, Raúl
dc.contributor.authorRaposo, Sara
dc.date.accessioned2021-07-05T08:41:02Z
dc.date.available2023-05-01T00:30:15Z
dc.date.issued2021-06
dc.description.abstractMicroalgae have been considered third generation feedstock for biofuel production based on the expectation that large amounts of algal biomass can be cultivated at an acceptable cost. Transformation of biomass into ethanol requires a saccharification step, where complex carbohydrates are broken down by hydrolysis into sugars that can be fermented to bioethanol. Carbohydrate mobilization is hampered by the recalcitrance of the cell envelope of microalgal cells, because complex structural polysaccharides are difficult to depolymerize and make internal carbohydrate reserves inaccessible to hydrolysis. Saccharification can be accomplished by either acidic hydrolysis, enzymatic treatment or a combination of both. The present work focused on the chemo-enzymatic hydrolysis of lyophilized biomass of different microalgae and subsequent fermentation of hydrolysates with higher reducing sugar content. A chemo-enzymatic hydrolysis strategy was defined, consisting of an acid pretreatment carried out at high pressure and temperature, followed by incubation with Amyloglucosidase and finally by incubation with alpha-Amylase, the opposite order of the conventional use of these enzymes. An increase of reducing sugar yield of about one third was observed, and this strategy was successfully applied to a broad group of microalgae, resulting in maximum release yields of at least 34.0 +/- 1.0 g total reducing sugar/100 g dry biomass. For bioethanol production studies, the microalgae hydrolysates of Chlorella sorokiniana, Tetraselmis sp. (Necton) and Skeletonema sp. were selected according to their high reducing sugar content. High ethanol production was achieved with all hydrolysates, with ethanol yields close to the theoretical maximum and the highest ethanol concentrations so far reported under comparable conditions. Chlorella sorokiniana stood out as the best hydrolysate for ethanol production, with an ethanol yield of 0.464 +/- 0.013 g/g reducing sugar and ethanol productivity of 0.344 +/- 0.020 g/L.h.pt_PT
dc.description.sponsorshipVA-POCTEP-2014-20200055_ALGARED_PLUS_5_E, UID/00350/2020, 0055 ALGARED + 5 Ept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.algal.2021.102329pt_PT
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/16720
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectMicroalgal biomass hydrolysispt_PT
dc.subjectFermentable sugars releasept_PT
dc.subjectAlpha-Amylasept_PT
dc.subjectAmyloglucosidasept_PT
dc.subjectEnzyme addition strategypt_PT
dc.subjectAlcoholic fermentationpt_PT
dc.titleAlternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage102329pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume56pt_PT
person.familyNameConstantino
person.familyNameRodrigues
person.familyNameJorge de Barros
person.familyNameRaposo
person.givenNameAna
person.givenNameBrígida
person.givenNameRaúl José
person.givenNameSara
person.identifierE-6295-2012
person.identifier.ciencia-id631C-A162-3F1E
person.identifier.ciencia-idF115-15E6-2A2A
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0002-9968-3512
person.identifier.orcid0000-0001-9495-0813
person.identifier.orcid0000-0002-6628-1437
person.identifier.orcid0000-0001-5344-7349
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7801639810
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication466ef81c-c32f-4829-a96e-97d37c157fc0
relation.isAuthorOfPublication99ea34cc-7325-4ae7-b288-31d7e43ab28f
relation.isAuthorOfPublication053d1546-401f-4fdb-9ea9-156619bf16eb
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication.latestForDiscovery466ef81c-c32f-4829-a96e-97d37c157fc0

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