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Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics

2010Journal article Restricted access
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dc.contributor.authorChora, Suze
dc.contributor.authorMcDonagh, Brian
dc.contributor.authorSheehan, David
dc.contributor.authorStarita-Geribaldi, Mireille
dc.contributor.authorRoméo, Michèle
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-04-30T12:53:41Z
dc.date.available2020-04-30T12:53:41Z
dc.date.issued2010
dc.description.abstractUbiquitination and carbonylation of proteins were investigated in the gill and digestive gland of Ruditapes decussatus exposed to NP (nonylphenol) (100 μgL(-1)) using redox proteomics. After 21 d of exposure, clams were dissected and cytosolic proteins of both tissues separated by 2DE SDS-PAGE. Protein expression profiles were tissue-dependent and differently affected by NP exposure. Ubiquitination and carbonylation were also tissue-specific. NP exposure induced significantly more ubiquitinated proteins in gills than in digestive glands, compared to controls. Digestive gland showed a significant higher number of carbonylated proteins than gills after NP exposure. Protein ubiquitination and carbonylation are therefore independent processes. Results showed that NP exposure generated ROS in gill and digestive gland of R. decussatus that significantly altered the proteome. Results also highlighted the advantage of using redox proteomics in the assessment of protein ubiquitination and carbonylation, which may be markers of oxidative stress in R. decussatus.pt_PT
dc.description.sponsorshipIrish Research Council for Science Engineering and Technologypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.chemosphere.2010.09.038pt_PT
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.1/13819
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationESTUDO DO EFEITO DE CONTAMINANTES QUÍMICOS NUMA ESPÉCIE SENTINELA, A AMÊIJOA RUDITAPES DECUSSATUS ATRAVÉS DA ANÁLISE DA MODIFICAÇÃO DA EXPRESSÃO PROTEICA E GENÓMICA: APLICAÇÃO À MONITORIZAÇÃO DO AMBIENTE MARINHO
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0045653510010532?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBivalviapt_PT
dc.subjectOxidation-reductionpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectPhenolspt_PT
dc.subjectProtein carbonylationpt_PT
dc.subjectProteomept_PT
dc.subjectProteomicspt_PT
dc.subjectUbiquitinationpt_PT
dc.subjectWater pollutantspt_PT
dc.subjectChemicalpt_PT
dc.subjectCarbonylationpt_PT
dc.subjectEcotoxicologypt_PT
dc.subjectNonylphenolpt_PT
dc.subjectRuditapes decussatuspt_PT
dc.titleUbiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomicspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleESTUDO DO EFEITO DE CONTAMINANTES QUÍMICOS NUMA ESPÉCIE SENTINELA, A AMÊIJOA RUDITAPES DECUSSATUS ATRAVÉS DA ANÁLISE DA MODIFICAÇÃO DA EXPRESSÃO PROTEICA E GENÓMICA: APLICAÇÃO À MONITORIZAÇÃO DO AMBIENTE MARINHO
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F19477%2F2004/PT
oaire.citation.endPage1217pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1212pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume81pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublicationa7f5f67d-82b6-475d-ba23-0955ed06e185
relation.isProjectOfPublication.latestForDiscoverya7f5f67d-82b6-475d-ba23-0955ed06e185

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