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Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification

2013Journal article Open access
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dc.contributor.authorBrunes, T. O.
dc.contributor.authorvan de Vliet, M. S.
dc.contributor.authorLopes, S.
dc.contributor.authorAlexandrino, J.
dc.contributor.authorHaddad, C. F. B.
dc.contributor.authorSequeira, F.
dc.date.accessioned2018-12-07T14:58:30Z
dc.date.available2018-12-07T14:58:30Z
dc.date.issued2013
dc.description.abstractTwelve polymorphic tetranucleotide microsatellite loci were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri, an endemic species of the Brazilian Atlantic forest. These loci were screened in 25 individuals from two populations of the Minas Gerais State (Carangola and Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (mean = 8). Observed and expected heterozygosities ranged from 0.25 to 0.92 and 0.56 to 0.92, respectively. Evidence for both the presence of null alleles and Hardy-Weinberg equilibrium deviations were found in loci Phybu4, Phybu17, and Phybu21. Genotypic disequilibrium for each pair of loci across populations was not significant. Cross-species amplification was successful for 11 of the 12 developed loci for the sister-species, P. bahiana. These microsatellites will be important for future fine-scale population structure analyses.
dc.identifier.doi10.4238/2013.January.30.10
dc.identifier.issn1676-5680
dc.identifier.urihttp://hdl.handle.net/10400.1/12055
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFunpec-Editora
dc.relationNuclear gene genealogies, landscape genetics and GIS: developing an integrated approach to test hypotheses on the biogeography of Iberian herpetofauna
dc.relationANÁLISE EVOLUTIVA DO COMPLEXO DE ESPÉCIES DE ANUROS NEOTROPICAIS PHYLLOMEDUSA BURMEISTERI: FILOGEOGRAFIA, HIBRIDIZAÇÃO E ESPECIAÇÃO
dc.relationBIOGEOGRAPHY AND DIVERSIFICATION OF THE BRAZILIAN ATLANTIC FOREST ENDEMIC TREEFROG SPECIES GROUP PHYLLOMEDUSA BURMEISTERI ANURA: PHYLLOMEDUSINAE: AN INTEGRATIVE APPROACH COMBINING MOLECULAR ANALYSES AND DISTRIBUTION MODELLING
dc.subjectBrazilian Atlantic Forest
dc.subjectConservation
dc.titleCharacterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNuclear gene genealogies, landscape genetics and GIS: developing an integrated approach to test hypotheses on the biogeography of Iberian herpetofauna
oaire.awardTitleANÁLISE EVOLUTIVA DO COMPLEXO DE ESPÉCIES DE ANUROS NEOTROPICAIS PHYLLOMEDUSA BURMEISTERI: FILOGEOGRAFIA, HIBRIDIZAÇÃO E ESPECIAÇÃO
oaire.awardTitleBIOGEOGRAPHY AND DIVERSIFICATION OF THE BRAZILIAN ATLANTIC FOREST ENDEMIC TREEFROG SPECIES GROUP PHYLLOMEDUSA BURMEISTERI ANURA: PHYLLOMEDUSINAE: AN INTEGRATIVE APPROACH COMBINING MOLECULAR ANALYSES AND DISTRIBUTION MODELLING
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-BDE%2F60911%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F27134%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F61689%2F2009/PT
oaire.citation.endPage247
oaire.citation.issue1
oaire.citation.startPage242
oaire.citation.titleGenetics and Molecular Research
oaire.citation.volume12
oaire.fundingStreamPOCI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isProjectOfPublicationddc0c114-6cb8-4ec1-b366-9566253bb2a0
relation.isProjectOfPublication4f977c57-70c3-41fc-a7e2-8dc2d12f1865
relation.isProjectOfPublication.latestForDiscoverybc7d7305-3e0c-4ba8-b076-eba80aebec4b

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