Repository logo
 
Publication

Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken

2010Journal article Open access
Simple item page
dc.contributor.authorPinheiro, Pedro L. C.
dc.contributor.authorCardoso, João CR
dc.contributor.authorFuentes, J.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-10-22T12:53:28Z
dc.date.available2014-10-22T12:53:28Z
dc.date.issued2010
dc.description.abstractBackground: Parathyroid hormone (PTH) and PTH-related peptide (PTHrP) belong to a family of endocrine factors that share a highly conserved N-terminal region (amino acids 1-34) and play key roles in calcium homeostasis, bone formation and skeletal development. Recently, PTH-like peptide (PTH-L) was identified in teleost fish raising questions about the evolution of these proteins. Although PTH and PTHrP have been intensively studied in mammals their function in other vertebrates is poorly documented. Amphibians and birds occupy unique phylogenetic positions, the former at the transition of aquatic to terrestrial life and the latter at the transition to homeothermy. Moreover, both organisms have characteristics indicative of a complex system in calcium regulation. This study investigated PTH family evolution in vertebrates with special emphasis on Xenopus and chicken. Results: The PTH-L gene is present throughout the vertebrates with the exception of placental mammals. Gene structure of PTH and PTH-L seems to be conserved in vertebrates while PTHrP gene structure is divergent and has acquired new exons and alternative promoters. Splice variants of PTHrP and PTH-L are common in Xenopus and chicken and transcripts of the former have a widespread tissue distribution, although PTH-L is more restricted. PTH is widely expressed in fish tissue but from Xenopus to mammals becomes largely restricted to the parathyroid gland. The N-terminal (1-34) region of PTH, PTHrP and PTH-L in Xenopus and chicken share high sequence conservation and the capacity to modify calcium fluxes across epithelia suggesting a conserved role in calcium metabolism possibly via similar receptors. Conclusions: The parathyroid hormone family contains 3 principal members, PTH, PTHrP and the recently identified PTH-L. In teleosts there are 5 genes which encode PTHrP (2), PTH (2) and PTH-L and in tetrapods there are 3 genes (PTHrP, PTH and PTH-L), the exception is placental mammals which have 2 genes and lack PTH-L. It is hypothesized that genes of the PTH family appeared at approximately the same time during the vertebrate radiation and evolved via gene duplication/deletion events. PTH-L was lost from the genome of eutherian mammals and PTH, which has a paracrine distribution in lower vertebrates, became the product of a specific endocrine tissue in Amphibia, the parathyroid gland. The PTHrP gene organisation diverged and became more complex in vertebrates and retained its widespread tissue distribution which is congruent with its paracrine nature.por
dc.description.sponsorshipThis research was funded by European Social Fund and the Portuguese National Science Foundation (project PTDC/CVT/66735/2006 and fellowships SFRH/BD/30881/2006 and BPD/30560/2006, respectively, to PLCP and JCRC) and Ceratonia prize (University of Algarve/Caixa Geral de Depósitos) to AVMC.por
dc.identifier.citationPedro LC Pinheiro, João CR Cardoso, Ana S Gomes, Juan Fuentes, Deborah M Power, Adelino VM Canário, "Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken" in BMC Evolutionary Biology 2010, 10:373.por
dc.identifier.doihttp://dx.doi.org/ 10.1186/1471-2148-10-373
dc.identifier.issn1471-214
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/5403
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBioMed Centralpor
dc.relation.publisherversionhttp://www.biomedcentral.com/1471-2148/10/373por
dc.subjectEvolutionary Biologypor
dc.subjectParathyroid hormonepor
dc.titleGene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chickenpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14por
oaire.citation.issue1por
oaire.citation.startPage1por
oaire.citation.titleBMC Evolutionary Biologypor
oaire.citation.volume10por
person.familyNameCardoso
person.familyNameFuentes
person.familyNamePower
person.familyNameCanario
person.givenNameJoão
person.givenNameJuan
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier14332
person.identifier392998
person.identifier143624
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-4151-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication1b670c84-15e3-4776-8871-50f9eb0eed0d
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken.pdf
Size:
1.04 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.49 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)