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Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish

2019-01Journal article Open access
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dc.contributor.authorI, Fernández
dc.contributor.authorFernandes, Jorge M. O.
dc.contributor.authorRoberto, Vânia
dc.contributor.authorKopp, Martina
dc.contributor.authorOliveira, Catarina
dc.contributor.authorRiesco, Marta F.
dc.contributor.authorDias, Jorge
dc.contributor.authorCox, Cymon J.
dc.contributor.authorLeonor Cancela, M.
dc.contributor.authorCabrita, Elsa
dc.contributor.authorGavaia, Paulo
dc.date.accessioned2020-07-24T10:53:32Z
dc.date.available2020-07-24T10:53:32Z
dc.date.issued2019-01
dc.description.abstractBackground: Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood. Methods: The potential role of VK on reproduction through nutritional supplementation in Senegalese sole (Solea senegalensis) was assessed by gonadal maturation and 11-ketosterone, testosterone and estriol plasma levels when fed with control or VK supplemented (1250 mg kg(-1) of VK,) diets along a six month trial. At the end, sperm production and quality (viability and DNA fragmentation) were evaluated. Circulating small non-coding RNAs (sncRNAs) in blood plasma from males were also studied through RNA-Seq. Results: Fish fed with dietary VK supplementation had increased testosterone levels and lower sperm DNA fragmentation. SncRNAs from blood plasma were found differentially expressed when nutritional and sperm quality conditions were compared. PiR-675//676//4794//5462 and piR-74614 were found up-regulated in males fed with dietary VK supplementation. Let-7g, let-7e(18nt), let-7a-1, let-7a-3//7a-2//7a-1, let-7e(23nt) and piR-675//676//4794//5462 were found to be up-regulated and miR-146a and miR-146a-1//146a-2//146a-3 down-regulated when fish with low and high sperm DNA fragmentation were compared. Bioinformatic analyses of predicted mRNAs targeted by sncRNAs revealed the potential underlying pathways. Conclusions: VK supplementation improves fish gonad maturation and sperm quality, suggesting an unexpected and complex regulation of the nutritional status and reproductive performance through circulating sncRNAs. General significance: The use of circulating sncRNAs as reliable and less-invasive physiological biomarkers in fish nutrition and reproduction has been unveiled.
dc.description.sponsorshipEuropean Regional Development Funds (ERDF) through the Portuguese "Programa Operational" (PROMAR)European Union (EU) [31-03-05-FEP-0073]
dc.description.sponsorshipCOST Office (Food and Agriculture COST Action) [FA1205: AQUAGAMETE]
dc.description.sponsorshipnetwork LARVAplus "Estrategias de desarrollo y mejora de la production de larvas de peces en Iberoamerica" - Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo (CYTED) [117RT0521]
dc.description.sponsorshipCYTED [318RT0549]
dc.description.sponsorship[CCMAR/SC/BPD/09/2016]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.bbagen.2018.09.017
dc.identifier.issn0304-4165
dc.identifier.issn1872-8006
dc.identifier.urihttp://hdl.handle.net/10400.1/14504
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
dc.relationCHRONO-REPRODUCTION IN TELEOST FISH: MECHANISMS OF SYNCHRONIZATION TO ENVIRONMENTAL CYCLES
dc.subjectSole solea-senegalensis
dc.subjectSex steroid-levels
dc.subjectPlasma-levels
dc.subjectGene-expression
dc.subjectSperm quality
dc.subjectDna-damage
dc.subjectPerformance
dc.subjectEnzyme
dc.subjectSupplementation
dc.subjectMetabolism
dc.titleCirculating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
oaire.awardTitleCHRONO-REPRODUCTION IN TELEOST FISH: MECHANISMS OF SYNCHRONIZATION TO ENVIRONMENTAL CYCLES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F82049%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F63933%2F2009/PT
oaire.citation.endPage51
oaire.citation.issue1
oaire.citation.startPage39
oaire.citation.titleBBA-GeneralSubjects
oaire.citation.volume1863
oaire.fundingStream5876
person.familyNameFernández Monzón
person.familyNameRoberto
person.familyNameOliveira
person.familyNameCox
person.familyNameCancela
person.familyNameCabrita
person.familyNameGavaia
person.givenNameIgnacio Valentín
person.givenNameVânia
person.givenNameCatarina
person.givenNameCymon
person.givenNameM. Leonor
person.givenNameElsa
person.givenNamePaulo
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person.identifier.scopus-author-id26645697000
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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