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Metallothionein in the freshwater gastropod Melanopsis dufouri chronically exposed to cadmium: A methodological approach

2010Journal article Restricted access
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dc.contributor.authorUreña, Rocío
dc.contributor.authorBebianno, Maria João
dc.contributor.authordel Ramo, Jose
dc.contributor.authorTorreblanca, Amparo
dc.date.accessioned2020-04-30T15:32:13Z
dc.date.available2020-04-30T15:32:13Z
dc.date.issued2010
dc.description.abstractPrevious studies have demonstrated that the use of differential pulse polarography (DPP) for metallothionein (MT) determination in marine gastropod tissues, particularly the digestive gland, requires taking into account the presence of heat-stable high molecular weight compounds that exhibit polarographic signal. In the present paper, similar compounds were identified in tissues from the freshwater snail Melanopsis dufouri which also interfere with MT determination by DPP and, due to their silver binding capacity, also interfere in the silver assay for MT quantification. Ultrafiltration seems to be effective in removing these high molecular weight compounds from heat-denatured homogenate supernatant allowing direct MT quantification by DPP. A fully validated procedure for metallothionein determination in M. dufouri is described. In spite of a considerable accumulation of cadmium in the visceral complex of M. dufouri following exposure to 100 microg CdL(-1) for 8 weeks (up to 37 microgg(-1)) only a small increase in MT concentration was found.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ecoenv.2010.02.010pt_PT
dc.identifier.issn0147-6513
dc.identifier.urihttp://hdl.handle.net/10400.1/13820
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademic Presspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectCadmiumpt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectSnailspt_PT
dc.subjectToxicity testspt_PT
dc.subjectWater pollutantspt_PT
dc.subjectChronicpt_PT
dc.subjectChemicalpt_PT
dc.titleMetallothionein in the freshwater gastropod Melanopsis dufouri chronically exposed to cadmium: A methodological approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage787pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage779pt_PT
oaire.citation.titleEcotoxicology and Environmental Safetypt_PT
oaire.citation.volume73pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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