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Identification and analysis of teleost slow muscle troponin T (sTnT) and intronless TnT genes

2005Journal article Restricted access
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dc.contributor.authorCampinho, Marco António
dc.contributor.authorPower, Deborah
dc.contributor.authorSweeney, Glen E.
dc.date.accessioned2014-10-23T09:57:02Z
dc.date.available2014-10-23T09:57:02Z
dc.date.issued2005
dc.description.abstractIn the present study cDNA clones representing two slow skeletal muscle troponin T genes (sTnT1sb and sTnT2sb) in the sea bream (Sparus auratus), an important aquaculture species, were isolated and characterised. A third, intronless, TnT gene (iTnTsb), which is an apparent orthologue of a previously described zebrafish TnT, was also isolated. In adult sea bream sTnT expression was restricted to red muscle and, using northern blotting, a single low abundance transcript was identified for sTnT1sb (1260 nucleotides) and a single high abundance transcript was identified for sTnT2sb (1000 nucleotides). In contrast, iTnTsb is predominantly expressed in adult fast muscle. All three TnT genes are also expressed during larval development. Phylogenetic analysis of sea bream sTnT proteins to identify maximum parsimony showed that iTnTsb, sTnT1sb and sTnT2sb each cluster in independent groups. sTnT1sb clustered with other vertebrate sTnTs, while sTnT2 clustered with a group of fish specific sequences (from Fugu rubripes, Oryzia latipes and Salmo trutta). The teleost sTnT2 and iTnT each constitute new, apparently teleost specific, TnT groups. Analysis of the corresponding Fugu scaffold indicates that sTnT2sb is encoded by a gene with twelve exons. The two sTnT cDNAs isolated in sea bream probably arose by duplication of an ancestral gene, and iTnT by reverse transcription. It remains to be established if the encoded proteins have different structural and mechanistic roles in fish muscle.por
dc.description.sponsorshipThis work was funded by European Union project CT 96-1442 and Portuguese Ministry of Science and Technology—Plurinual funding to CCMAR. MA Campinho is funded by Portuguese Ministry of Science and Technology (SFRH/BD/6133/2001). We thank Dr. Juan Miguel Mancera (University of Cadiz, Spain) for kindly providing the sea bream embryos used in this work.por
dc.identifier.citationM.A. Campinho, Deborah Power, G.E. Sweeney, "Identification and analysis of teleost slow muscle troponin T (sTnT) and intronless TnT genes" in Gene 361 (2005) 67 – 79.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.gene.2005.07.003
dc.identifier.issn0378-1119
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5422
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectTroponin-Tpor
dc.subjectSea breampor
dc.subjectSlow skeletal musclepor
dc.subjectGene organizationpor
dc.subjectGene functionpor
dc.titleIdentification and analysis of teleost slow muscle troponin T (sTnT) and intronless TnT genespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage79por
oaire.citation.startPage67por
oaire.citation.titleGenepor
oaire.citation.volume361por
person.familyNameCampinho
person.familyNamePower
person.givenNameMarco António
person.givenNameDeborah Mary
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-5238-0506
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id8938999600
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf8e8c466-6a6f-4ccf-b96d-34565fe53b6c
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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