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Evolution and diversity of alpha-carbonic anhydrases in the mantle of the Mediterranean mussel (Mytilus galloprovincialis)

2019Journal article Open access
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dc.contributor.authorCardoso, João CR
dc.contributor.authorFerreira, Vinicius
dc.contributor.authorZhang, Xushuai
dc.contributor.authorAnjos, Liliana
dc.contributor.authorFélix, Rute
dc.contributor.authorBatista, Frederico
dc.contributor.authorPower, Deborah
dc.date.accessioned2019-08-02T11:55:50Z
dc.date.available2019-08-02T11:55:50Z
dc.date.issued2019
dc.description.abstractThe α-carbonic anhydrases (α-CAs) are a large and ancient group of metazoan-specific enzymes. They generate bicarbonate from metabolic carbon dioxide and through calcium carbonate crystal formation play a key role in the regulation of mineralized structures. To better understand how α-CAs contribute to shell mineralization in the marine Mediterranean mussel (Mytilus galloprovincialis) we characterized them in the mantle. Phylogenetic analysis revealed that mollusc α-CA evolution was affected by lineage and species-specific events. Ten α-CAs were found in the Mediterranean mussel mantle and the most abundant form was named, MgNACR, as it grouped with oyster nacreins (NACR). Exposure of the Mediterranean mussel to reduced water salinity (18 vs 37 ppt), caused a significant reduction (p < 0.05) in mantle esterase activity and MgNACR transcript abundance (p < 0.05). Protonograms revealed multiple proteins in the mantle with α-CA hydratase activity and mapped to a protein with a similar size to that deduced for monomeric MgNACR. Our data indicate that MgNACR is a major α-CA enzyme in mantle and that by homology with oyster nacreins likely regulates mussel shell production. We propose that species-dependent α-CA evolution may contribute to explain the diversity of bivalve shell structures and their vulnerability to environmental changes.pt_PT
dc.description.sponsorshipAgência financiadora European Union 605051 Portuguese national funds from FCT - Foundation for Science and Technology UID/Multi/04326/2019 operational programmes CRESC Algarve 2020 and COMPETE 2020 through project EMBRC FCT UID/Multi/04326/2019 SFRH/BPD/79105/2011 SFRH/BPD/89811/2012 EMBRIC (European Union's Horizon 2020 research and innovation programme) 654008 CAPES, Brazil 88887.116718/2016-00 PT ALG-01-0145-FEDER-022121pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/ 10.1038/s41598-019-46913-2pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/12721
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleEvolution and diversity of alpha-carbonic anhydrases in the mantle of the Mediterranean mussel (Mytilus galloprovincialis)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79105%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage10400pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameCardoso
person.familyNameAnjos Guerreiro
person.familyNameFélix
person.familyNameBatista
person.familyNamePower
person.givenNameJoão
person.givenNameLiliana Isabel Tomé
person.givenNameRute
person.givenNameFrederico
person.givenNameDeborah Mary
person.identifier14332
person.identifierAAK-3800-2020
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-idCB1B-7DBC-6303
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-2848-3011
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.ridF-5651-2010
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id7003808684
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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