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Is gene transcription in mussel gills altered after exposure to Ag nanoparticles?

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dc.contributor.authorBebianno, Maria João
dc.contributor.authorGonzalez-Rey, Maria
dc.contributor.authorGomes, Tânia
dc.contributor.authorMattos, J. J.
dc.contributor.authorFlores-Nunes, F.
dc.contributor.authorBainy, A. C. D.
dc.date.accessioned2020-04-21T14:29:59Z
dc.date.available2020-04-21T14:29:59Z
dc.date.issued2015
dc.description.abstractNanotechnology is a rapid field of development with the enhancement of the production of different types of nanoparticles (NPs) applied in several industrial and commercial applications which increase the risk of their presence in the aquatic environment. Ag NPs have a wide application in everyday life products. However, there is concern about the exposure effects on aquatic organisms to these NPs. Therefore, this study aims to assess gene transcription alterations in mussels Mytilus galloprovincialis gills exposed for 2 weeks to Ag NPs (42 ± 10 nm, 10 μg.L(-1)). The genes were selected based on previous biomarkers and proteomic results and included superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), caspase 3/7-1 (CAS), cathepsin L (CATH), heat-shock protein 70 (HSP 70), cytochrome P450 4YA (CYP 4YA), the elongation factor (EF1), actin and α- tubulin. No significant changes in gene transcription profiles were observed after exposure of M. galloprovincialis to Ag NPs for 15 days. The lack of significant gene transcription responses is in light with previous results obtained for mussels exposed to these NPs and may be related to the fact that enzyme kinetics and relative abundance of proteins (increase of antioxidant enzymes and metalllothioneins (MTs) with the time of exposure) do not always directly reflect their relative mRNA levels. Nevertheless, their overall expression maintenance may signify that, at end of the exposure period (15 days), the transcription of the respective genes is no longer required, pointing out to a possible adaptation effect to nanoparticles or due to the levels of Ag NPs accumulated in this tissue at this exposure time. This study highlights that gene transcription application and role as an additional and/or alternative end point approach is important to understand the mode of action of these emergent contaminants in aquatic organisms. However, in future studies, the time window needs to be adjusted, as genes are likely to respond earlier to the nanoparticle exposure.pt_PT
dc.description.sponsorshipPhD Grant (SFRH/BD/41605/2007)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11356-015-5186-zpt_PT
dc.identifier.issn0944-1344
dc.identifier.urihttp://hdl.handle.net/10400.1/13737
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectGillspt_PT
dc.subjectMetal nanoparticlespt_PT
dc.subjectMytiluspt_PT
dc.subjectSilverpt_PT
dc.subjectTranscriptionpt_PT
dc.subjectGeneticpt_PT
dc.titleIs gene transcription in mussel gills altered after exposure to Ag nanoparticles?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAAC-AMB%2F121650%2F2010/PT
oaire.citation.endPage17433pt_PT
oaire.citation.issue22pt_PT
oaire.citation.startPage17425pt_PT
oaire.citation.titleEnvironmental Science and Pollution Researchpt_PT
oaire.citation.volume22pt_PT
oaire.fundingStreamCOMPETE
person.familyNameBebianno
person.familyNameGonzalez-Rey
person.familyNameGomes
person.givenNameMaria
person.givenNameMaria
person.givenNameTânia
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.orcid0000-0002-9343-8460
person.identifier.orcid0000-0001-6179-4543
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication182cc3b5-3e8d-4baf-9220-638978bef660
relation.isAuthorOfPublication71dcd70c-3a6a-45f7-bbe7-6b8a5346cc88
relation.isAuthorOfPublication.latestForDiscovery182cc3b5-3e8d-4baf-9220-638978bef660
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relation.isProjectOfPublication.latestForDiscovery4a1b04fa-ed2d-4fa6-ad48-19c74272bcc0

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