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Publicação
Matrix Gla protein gene expression and protein accumulation co-localize with cartilage distribution during development of the teleost fish Sparus aurata
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Orientador(es)
Resumo(s)
Matrix Gla protein (MGP) is a member of the family of extracellular mineral-binding Gla proteins, expressed in several tissues with high
accumulation in bone and cartilage. Although the precise molecular mechanism of action of this protein remains unknown, all available
evidence indicates that MGP plays a role as an inhibitor of mineralization. We investigated the sites of gene expression and protein
accumulation of MGP throughout development of the bony fish Sparus aurata, by in situ hybridization, Northern and RT-PCR Southern
hybridization, and immunohistochemistry. The results obtained were compared with the patterns of developmental appearance of cartilaginous
and mineralized structures in this species, identified by histological techniques and by detection of mRNA presence and protein
accumulation of osteocalcin (Bone Gla protein), a marker for osteoblasts known to accumulate in bone mineralized extracellular matrix.
Descrição
Palavras-chave
Matrix Gla Protein Development Bone and Cartilage appearance
Contexto Educativo
Citação
Pinto, J.P., Conceição, N., Gavaia, P.J., Cancela, M.L. 2003. Matrix Gla protein gene expression and protein accumulation co-localize with cartilage distribution during development of the teleost fish Sparus aurata. Bone 32: 201 –210.
Editora
Elsevier