Repository logo
 
Publication

Matrix Gla protein gene expression and protein accumulation co-localize with cartilage distribution during development of the teleost fish Sparus aurata

2003Journal article Unknown
Simple item page
dc.contributor.authorPinto, Jorge
dc.contributor.authorConceição, N.
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-25T12:16:30Z
dc.date.available2014-06-25T12:16:30Z
dc.date.issued2003
dc.date.updated2014-05-30T10:31:24Z
dc.description.abstractMatrix Gla protein (MGP) is a member of the family of extracellular mineral-binding Gla proteins, expressed in several tissues with high accumulation in bone and cartilage. Although the precise molecular mechanism of action of this protein remains unknown, all available evidence indicates that MGP plays a role as an inhibitor of mineralization. We investigated the sites of gene expression and protein accumulation of MGP throughout development of the bony fish Sparus aurata, by in situ hybridization, Northern and RT-PCR Southern hybridization, and immunohistochemistry. The results obtained were compared with the patterns of developmental appearance of cartilaginous and mineralized structures in this species, identified by histological techniques and by detection of mRNA presence and protein accumulation of osteocalcin (Bone Gla protein), a marker for osteoblasts known to accumulate in bone mineralized extracellular matrix.por
dc.description.sponsorshipThis work was partially funded by research grants (PRAXIS/BIA/469/94 and BIA/11159/98) from the Portuguese Science and Technology Foundation (FCT) and research funds from CCMAR/University of Algarve. We thank D. Simes for her contribution of polyclonal antibodies against fish MGP and osteocalcin. J.P. Pinto, N. Conceic¸a˜o, and P.J. Gavaia were the recipients of a postdoctoral fellowship (PRAXIS XXI/BPD/20229/99) and two PhD fellowships (PRAXIS BD/11567/97 and PRAXIS BD/19665/ 99), respectively.por
dc.identifier.citationPinto, J.P., Conceição, N., Gavaia, P.J., Cancela, M.L. 2003. Matrix Gla protein gene expression and protein accumulation co-localize with cartilage distribution during development of the teleost fish Sparus aurata. Bone 32: 201 –210.por
dc.identifier.doihttp://dx.doi.org/10.1016/S8756-3282(02)00981-X
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4493
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectMatrix Gla Proteinpor
dc.subjectDevelopmentpor
dc.subjectBone and Cartilage appearancepor
dc.titleMatrix Gla protein gene expression and protein accumulation co-localize with cartilage distribution during development of the teleost fish Sparus auratapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage210por
oaire.citation.startPage201por
oaire.citation.titleBonepor
oaire.citation.volume32por
person.familyNamePinto
person.familyNameGavaia
person.familyNameCancela
person.givenNameJorge
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0003-1501-458X
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridJ-7827-2013
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id8656187600
person.identifier.scopus-author-id6507104377
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication84f2bfd8-21d5-42f9-ab9b-510e975ce7f1
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery84f2bfd8-21d5-42f9-ab9b-510e975ce7f1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Matrix Gla protein gene expression and protein accumulation .pdf
Size:
611.39 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)