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Five gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationships

2005Journal article Restricted
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dc.contributor.authorMoncaut, Natalia P.
dc.contributor.authorSomoza, G. M.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-12T16:30:41Z
dc.date.available2014-05-12T16:30:41Z
dc.date.issued2005
dc.date.updated2014-05-09T08:57:36Z
dc.description.abstractGonadotrophin-releasing hormone (GnRH) is the main neurohormone controlling gonadotrophin release in all vertebrates, and in teleost fish also of growth hormone and possibly of other adenohypophyseal hormones. Over 20 GnRHs have been identified in vertebrates and protochoordates and shown to bind cognate G-protein couple receptors (GnRHR). We have searched the puffer fish, Fugu rubripes, genome sequencing database, identified five GnRHR genes and proceeded to isolate the corresponding complementary DNAs in European sea bass, Dicentrachus labrax. Phylogenetic analysis clusters the European sea bass, puffer fish and all other vertebrate receptors into two main lineages corresponding to the mammalian type I and II receptors. The fish receptors could be subdivided in two GnRHR1 (A and B) and three GnRHR2 (A, B and C) subtypes. Amino acid sequence identity within receptor subtypes varies between 70 and 90% but only 50–55% among the two main lineages in fish. All European sea bass receptor mRNAs are expressed in the anterior and mid brain, and all but one are expressed in the pituitary gland. There is differential expression of the receptors in peripheral tissues related to reproduction (gonads), chemical senses (eye and olfactory epithelium) and osmoregulation (kidney and gill). This is the first report showing five GnRH receptors in a vertebrate species and the gene expression patterns support the concept that GnRH and GnRHRs play highly diverse functional roles in the regulation of cellular functions, besides the ‘‘classical’’ role of pituitary function regulation.por
dc.identifier.citationMoncaut, N.; Somoza, G. M.; Power, D. M.; Canario, A. V. M.Five gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationships, Journal of Molecular Endocrinology, 34, 3, 767-779, 2005por
dc.identifier.issn0952-5041
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3871
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSociety for Endocrinologypor
dc.titleFive gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationshipspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage779por
oaire.citation.startPage767por
oaire.citation.titleJournal of Molecular Endocrinologypor
oaire.citation.volume34por
person.familyNamePower
person.familyNameCanario
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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