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Characterization of Sparus aurata osteonectin cDNA and in silico analysis of protein conserved features: Evidence for more than one osteonectin in Salmonidae

2005-05Journal article Restricted access
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dc.contributor.authorLaizé, Vincent
dc.contributor.authorPombinho, A. R.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-14T10:20:44Z
dc.date.available2014-06-14T10:20:44Z
dc.date.issued2005-05
dc.date.updated2014-06-03T09:58:47Z
dc.description.abstractOsteonectin is a matricellular protein involved in various cellular mechanisms but its exact function remains unclear despite numerous studies. We present here the cloning of Sparus aurata partial osteonectin cDNA and the reconstruction of 15 other sequences from both vertebrates and invertebrates, almost doubling the set of available sequences (a total of 35 sequences is now available). Taking advantage of the resulting large amount of data, we have created multiple sequence alignments and identified osteonectin putative conserved features (intra- and inter-disulfide bonds, collagen- and calcium-binding domains and phosphorylation sites) likely to be important for protein structure and function. This work also provides the first evidence for the presence of more than one osteonectin in some species. Finally, S. aurata osteonectin gene expression has been shown to initiate during larval development shortly after gastrulation, and to be high in bone-derived cell lines while down-regulated during extracellular matrix mineralization, further emphasizing the important role of osteonectin in skeletal development and bone formation.por
dc.identifier.citationLaizé V; Pombinho AR; Cancela ML. Characterization of Sparus aurata osteonectin cDNA and in silico analysis of protein conserved features: Evidence for more than one osteonectin in Salmonidae, Biochimie, 87, 5, 411-420, 2005.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.biochi.2005.01.008
dc.identifier.issn0300-9084
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4317
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0300908405000143por
dc.subjectOsteonectinpor
dc.subjectSparus aurata (teleostei)por
dc.subjectSPARCpor
dc.subjectExtracellular matrixpor
dc.subjectFish cell linepor
dc.subjectLarval developmentpor
dc.titleCharacterization of Sparus aurata osteonectin cDNA and in silico analysis of protein conserved features: Evidence for more than one osteonectin in Salmonidaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage420por
oaire.citation.issue5por
oaire.citation.startPage411por
oaire.citation.titleBiochimiepor
oaire.citation.volume87por
person.familyNameLaizé
person.familyNameCancela
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

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